| In-gel expression and in situ immobilization of proteins for generation of three dimensional protein arrays in a hydrogel matrix. | |
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MedLine Citation:
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PMID: 23303405 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A method has been developed for the direct conversion of DNA arrays into three dimensional protein arrays on a hydrogel matrix. An agarose gel embedded with bacterial protein synthesis machinery was used as the DNA-programmable expression gel matrix for the in situ translation of genes on a DNA array. Upon incubation of the expression gel matrix cast on a DNA array, protein synthesis took place at the interface of the two surfaces and the cell-free synthesized proteins were deposited on the gel matrix surrounding the corresponding DNA spots. Diffusional dilution of the expressed proteins was minimized by modifying the agarose with Ni-NTA moieties. This procedure resulted in the generation of localized protein spots with confined radii. The developed approach not only simplifies the procedures typically used for the preparation of protein arrays but it also provides conditions for the loading of higher amounts of proteins on the array while retaining their structural integrity and functionality over extended time periods. |
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Authors:
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Ju-Young Byun; Kyung-Ho Lee; Ka-Young Lee; Min-Gon Kim; Dong-Myung Kim |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-10 |
Journal Detail:
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Title: Lab on a chip Volume: - ISSN: 1473-0189 ISO Abbreviation: Lab Chip Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea. dmkim@cnu.ac.kr. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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