Document Detail


In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
MedLine Citation:
PMID:  23533145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expressed prostatic secretions (EPS) are proximal fluids of the prostate that are increasingly being utilized as a clinical source for diagnostic and prognostic assays for prostate cancer (PCa). These fluids contain an abundant amount of microvesicles reflecting the secretory function of the prostate gland, and their protein composition remains poorly defined in relation to PCa. Using expressed prostatic secretions in urine (EPS-urine), exosome preparations were characterized by a shotgun proteomics procedure. In pooled EPS-urine exosome samples, ~900 proteins were detected. Many of these have not been previously observed in the soluble proteome of EPS generated by our labs or other related exosome proteomes. We performed systematic comparisons of our data against previously published, prostate-related proteomes, and global annotation analyses to highlight functional processes within the proteome of EPS-urine derived exosomes. The acquired proteomic data have been deposited to the Tranche repository and will lay the foundation for more extensive investigations of PCa derived exosomes in the context of biomarker discovery and cancer biology.
Authors:
Simona Principe; E Ellen Jones; Yunee Kim; Ankit Sinha; Julius O Nyalwidhe; Jasmin Brooks; O John Semmes; Dean A Troyer; Raymond S Lance; Thomas Kislinger; Richard R Drake
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-04-23
Journal Detail:
Title:  Proteomics     Volume:  13     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-06-04     Completed Date:  2013-12-26     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1667-71     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Descriptor/Qualifier:
Case-Control Studies
Exosomes / metabolism,  secretion*
Humans
Male
Prostatic Neoplasms / secretion*,  urine
Proteinuria / urine
Proteome / isolation & purification,  metabolism,  secretion*
Grant Support
ID/Acronym/Agency:
MOP-9772//Canadian Institutes of Health Research; R01 CA135087/CA/NCI NIH HHS; R01CA135087/CA/NCI NIH HHS; R21 CA137704/CA/NCI NIH HHS; R21CA137704/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Proteome
Comments/Corrections

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