Document Detail


In-depth analysis of the Magnaporthe oryzae conidial proteome.
MedLine Citation:
PMID:  23039028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The filamentous fungus Magnaporthe oryzae (M. oryzae) is the causative agent of rice blast disease and presents a significant threat to worldwide rice production. To establish the groundwork for future research on the pathogenic development of M. oryzae, a global proteomic study of conidia was performed. The filter aided sample preparation method (FASP) and anion StageTip fractionation combined with long, optimized shallow 210 min nanoLC gradients prior to mass spectrometry analysis on an Orbitrap XL was applied, which resulted in a doubling of protein identifications in comparison to our previous GeLC analysis. Herein, we report the identification of 2912 conidial proteins at a 1% protein false discovery rate (FDR) and we present the most extensive study performed on M. oryzae conidia to date. A similar distribution between identified proteins and the predicted proteome was observed when subcellular localization analysis was performed, suggesting the detected proteins build a representative portion of the predicted proteome. A higher percentage of cytoplasmic proteins (associated with translation, energy, and metabolism) were observed in the conidial proteome relative to the whole predicted proteome. Conversely, nuclear and extracellular proteins were less well represented in the conidial proteome. Further analysis by gene ontology revealed biological insights into identified proteins important for central metabolic processes and the physiology of conidia.
Authors:
Emine Gokce; William L Franck; Yeonyee Oh; Ralph A Dean; David C Muddiman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-10-29
Journal Detail:
Title:  Journal of proteome research     Volume:  11     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-11     Completed Date:  2013-05-23     Revised Date:  2013-12-12    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5827-35     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleus / chemistry
Chemical Fractionation / methods*
Chromatography, Liquid / methods
Cytoplasm / chemistry
Fungal Proteins / analysis*,  chemistry
Magnaporthe / chemistry*
Mass Spectrometry / methods
Nanotechnology
Nuclear Proteins / analysis,  chemistry
Peptides / analysis,  chemistry
Proteome / analysis*,  chemistry
Reproducibility of Results
Sensitivity and Specificity
Spores, Fungal / chemistry*
Time Factors
Grant Support
ID/Acronym/Agency:
T32 AI052080/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Fungal Proteins; 0/Nuclear Proteins; 0/Peptides; 0/Proteome
Comments/Corrections

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