Document Detail


In the adult mesenchymal stem cell population, source gender is a biologically relevant aspect of protective power.
MedLine Citation:
PMID:  17689688     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Acute treatment with bone marrow mesenchymal stem cells (MSC) reduces myocardial infarct size by multiple mechanisms, including the paracrine release of protective growth factors. Female MSCs produce more growth factor when stressed; therefore, we hypothesized that myocardial protection provoked by female MSCs would be greater than that elicited by male MSCs. METHODS: Hearts were subjected to 25 min of warm global ischemia, 40 min of reperfusion, and randomly assigned into one of three groups: (1) vehicle treated; (2) male MSC treated; and (3) female MSC treated. Myocardial function was continuously recorded and in separate experiments, male and female MSC growth factor production was assessed by ELISA. RESULTS: All indices of functional recovery were significantly higher in the stem cell infused rat heart compared with control hearts. Interestingly, female MSC treated rat hearts demonstrated significantly greater recovery of left ventricular developed pressure, +dP/dT, and -dP/dT than male MSC treated hearts at end reperfusion. In addition, male MSCs produced significantly greater tumor necrosis factor alpha, and significantly less vascular endothelial growth factor than female MSCs. CONCLUSIONS: This study is the first to demonstrate that, in the adult mesenchymal population, source gender is a biologically relevant aspect of ultimate stem cell function in the heart.
Authors:
Paul R Crisostomo; Troy A Markel; Meijing Wang; Tim Lahm; Keith D Lillemoe; Daniel R Meldrum
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Surgery     Volume:  142     ISSN:  0039-6060     ISO Abbreviation:  Surgery     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-10     Completed Date:  2007-09-12     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  0417347     Medline TA:  Surgery     Country:  United States    
Other Details:
Languages:  eng     Pagination:  215-21     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, Indiana University School of Medicine, Indianapolis, Ind, USA. pcrisost@iupui.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Female
Male
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stem Cells / cytology,  metabolism*
Mice
Mice, Inbred C57BL
Myocardial Infarction / therapy*
Rats
Rats, Sprague-Dawley
Reperfusion Injury / therapy*
Sex Characteristics*
Tumor Necrosis Factor-alpha / metabolism
Vascular Endothelial Growth Factor A / metabolism
Grant Support
ID/Acronym/Agency:
C06 RR015481-01/RR/NCRR NIH HHS; F32HL085982/HL/NHLBI NIH HHS; R01GM070628/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Tumor Necrosis Factor-alpha; 0/Vascular Endothelial Growth Factor A

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