| In vivo and in vitro laser confocal microscopy to diagnose acanthamoeba keratitis. | |
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MedLine Citation:
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PMID: 20508505 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To determine the effectiveness of laser confocal microscopy in identifying Acanthamoeba cysts and trophozoites in the cornea of patients with Acanthamoeba keratitis (AK) and to evaluate its effectiveness in following AK after treatment. METHODS: The corneas of 9 patients clinically diagnosed with AK were monitored periodically with the Heidelberg Retina Tomograph II-Rostock Cornea Module (HRT II-RCM) to examine for Acanthamoeba cysts and trophozoites during the clinical course. RESULTS: Seven of 9 patients had positive corneal smears, and 5 of 9 patients had positive laboratory cultures. HRT II-RCM demonstrated the presence of highly reflective polygonal shadows with lower reflective borders in the cornea of all patients. In 1 patient, a highly reflective pleomorphic shadow with small less-reflective areas was detected inside the cell. The former finding resembled the image of Acanthamoeba cysts in culture as observed by HRT II-RCM, and the latter observation with that of Acanthamoeba trophozoites in culture. After treatment, the number of highly reflective inflammatory cells decreased and the number and morphology of the corneal epithelial cells with highly reflective nuclei recovered to normal levels. CONCLUSION: These results indicate that in vivo laser confocal microscopy can be a useful method to make a diagnosis and to follow patients with AK. |
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Authors:
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Atsushi Shiraishi; Toshihiko Uno; Naoko Oka; Yuko Hara; Masahiko Yamaguchi; Yuichi Ohashi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cornea Volume: 29 ISSN: 1536-4798 ISO Abbreviation: Cornea Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-27 Completed Date: 2010-10-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8216186 Medline TA: Cornea Country: United States |
Other Details:
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Languages: eng Pagination: 861-5 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology and Regenerative Medicine, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. shiraia@m.ehime-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acanthamoeba
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isolation & purification* Acanthamoeba Keratitis / diagnosis*, drug therapy Adolescent Adult Animals Anti-Infective Agents, Local / therapeutic use Chlorhexidine / therapeutic use Cornea / parasitology* Drug Therapy, Combination Female Humans Lasers / diagnostic use Male Microscopy, Confocal* Middle Aged Tomography Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Anti-Infective Agents, Local; 55-56-1/Chlorhexidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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