| In Vivo Systems Analysis Identifies Spatial and Temporal Aspects of the Modulation of TNF-{alpha}-Induced Apoptosis and Proliferation by MAPKs. | |
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MedLine Citation:
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PMID: 21427409 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Cellular responses to external stimuli depend on dynamic features of multipathway network signaling; thus, cell behavior is influenced in a complex manner by the environment and by intrinsic properties. Methods of multivariate systems analysis have provided an understanding of these convoluted effects, but only for relatively simplified examples in vitro. To determine whether such approaches could be successfully used in vivo, we analyzed the signaling network that determines the response of intestinal epithelial cells to tumor necrosis factor-α (TNF-α). We built data-driven, partial least-squares discriminant analysis (PLSDA) models based on signaling, apoptotic, and proliferative responses in the mouse small intestinal epithelium after systemic exposure to TNF-α. The extracellular signal-regulated kinase (ERK) signaling axis was a critical modulator of the temporal variation in apoptosis at different doses of TNF-α and of the spatial variation in proliferation in distinct intestinal regions. Inhibition of MEK, a mitogen-activated protein kinase kinase upstream of ERK, altered the signaling network and changed the temporal and spatial phenotypes consistent with model predictions. Our results demonstrate the dynamic, adaptive nature of in vivo signaling networks and identify natural, tissue-level variation in responses that can be deconvoluted only with quantitative, multivariate computational modeling. This study lays a foundation for the use of systems-based approaches to understand how dysregulation of the cellular network state underlies complex diseases. |
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Authors:
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Ken S Lau; Alwin M Juchheim; Kimberly R Cavaliere; Sarah R Philips; Douglas A Lauffenburger; Kevin M Haigis |
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Publication Detail:
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Type: Journal Article Date: 2011-03-22 |
Journal Detail:
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Title: Science signaling Volume: 4 ISSN: 1937-9145 ISO Abbreviation: Sci Signal Publication Date: 2011 |
Date Detail:
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Created Date: 2011-03-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101465400 Medline TA: Sci Signal Country: United States |
Other Details:
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Languages: eng Pagination: ra16 Citation Subset: IM |
Affiliation:
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1Molecular Pathology Unit, Center for Cancer Research, and Center for Systems Biology, Massachusetts General Hospital, Charlestown, MA 02129, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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