Document Detail

In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-β in the Pacific Oyster Crassostrea gigas.
MedLine Citation:
PMID:  22147255     Owner:  NLM     Status:  Publisher    
We investigated the role of oyster gonadal TGFβ (og-TGFβ) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGFβ, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGFβ dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated into the gonad, likely as part of the active resorption of degenerating germ cells. Conversely, males showed a normal phenotype at the cellular level, with spermatids and spermatozoids observed in the gonads of control and injected males. As a result, og-TGFβ appears to play an essential role in C. gigas germ cell development by functioning as an activator of germ cell proliferation in both male and female oysters and vitellogenesis in females.
Arnaud Huvet; Elodie Fleury; Charlotte Corporeau; Virgile Quillien; Jean Yves Daniel; Guillaume Riviere; Pierre Boudry; Caroline Fabioux
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-7
Journal Detail:
Title:  Marine biotechnology (New York, N.Y.)     Volume:  -     ISSN:  1436-2236     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892712     Medline TA:  Mar Biotechnol (NY)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
IFREMER, UMR Physiologie et Ecophysiologie des Mollusques Marins, Technopole Brest-Iroise, BP70, 292800, Plouzané, France,
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