| In vivo morphine treatment synergistically increases LPS-induced caspase activity in immune organs. | |
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MedLine Citation:
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PMID: 20390371 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Caspases are a family of proteins important for the elimination of infected cells through the induction of apoptosis as well as the initiation of inflammatory cytokines including IL-1β and IL-18. Morphine exposure to animals and/or cells has been associated with the induction of apoptosis. The most common practices of apoptosis detection have involved removing tissues from animal or humans and the analysis of apoptosis on cells or tissues. These methods can potentially induce spontaneous apoptosis that is unrelated to the actual treatment. The objective of this study was to develop an in vivo detection method for assessing caspase activity induced both by morphine directly and by morphine combined with lipopolysaccharide (LPS)-immune activation. Mice were administered saline, morphine, LPS, or a combination of morphine and LPS. Prior to sacrifice, mice were injected with a polycaspase-specific apoptosis detection probe to detect internal caspase activity in vivo. Results revealed that morphine alone did not directly induce caspase activity. However, morphine significantly enhanced the LPS-induced caspase activity in spleen, thymus, and bone marrow-derived immune cells. The use of a poly-caspase detection probe methodology to label caspase activity in vivo provides a powerful quantitative tool for the in vivo analysis of caspase activity. |
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Authors:
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Michael R Olin; Sabita Roy; Thomas Molitor |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology Volume: 5 ISSN: 1557-1904 ISO Abbreviation: J Neuroimmune Pharmacol Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-19 Completed Date: 2011-02-28 Revised Date: 2012-01-09 |
Medline Journal Info:
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Nlm Unique ID: 101256586 Medline TA: J Neuroimmune Pharmacol Country: United States |
Other Details:
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Languages: eng Pagination: 546-52 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA. olin0012@umn.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analgesics, Opioid
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pharmacology* Animals Apoptosis / drug effects* Bone Marrow Cells / drug effects Caspases / metabolism* Cell Separation Drug Synergism Flow Cytometry Fluorescent Dyes Immunohistochemistry / methods* Lipopolysaccharides / pharmacology* Lymphoid Tissue / drug effects Mice Mice, Inbred C57BL Mice, Knockout Morphine / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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DA011806/DA/NIDA NIH HHS; DA015349/DA/NIDA NIH HHS; DA022935/DA/NIDA NIH HHS; K02 DA015349-10/DA/NIDA NIH HHS; R01 DA012104-13/DA/NIDA NIH HHS; R01 DA12104/DA/NIDA NIH HHS; T32 DA07097/DA/NIDA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Analgesics, Opioid; 0/Fluorescent Dyes; 0/Lipopolysaccharides; 57-27-2/Morphine; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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