Document Detail

In Vivo Monitoring of Natural Killer Cell-dependent Clearance of Lung Metastasis Using Dynamic Positron Emission Tomography.
MedLine Citation:
PMID:  22351657     Owner:  NLM     Status:  In-Data-Review    
Immunohistological methods indicated a rapid onset of cellular defence shortly after seeding of mammary adenocarcinoma cells into the lungs of F344 rats. The purpose of the present study was to monitor natural killer (NK) cell-mediated effects on tumour cell clearance in vivo, in this model of lung metastasis using dynamic positron-emission tomography (dPET). MADB106 breast cancer cells were labelled with 2'-[(18)F]-2'-deoxy-D-glucose (FDG) then injected intravenously, after the F344 rats had been anaesthetized and placed in a PET scanner. NK cell-depleted and sham-treated control rats were investigated in parallel. The radioactivity per region of interest (ROI) over the lungs peaked at 60 s past injection and was followed by a slow decline over the observation time of 40 min in both groups. Statistical analysis using a linear mixed model revealed that release of radioactivity from tumour cells or tumour cell disintegration was significantly slower in animals after depletion of NK cells compared with controls. There was no significant tumour cell homing in organs other than the lungs. Early kinetics of tumour cells after injection were defined. PET with FDG was shown to be an adequate method to further investigate novel options for using cellular host defence mechanisms in cancer patients.
Carsten Kruschinski; Yoshihisa Hama; Thomas Skripuletz; Bernhard Vaske; Wolfram H Knapp; Reinhold E Schmidt; Reinhard Pabst; Stephan VON Hörsten; Michael Hofmann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  In vivo (Athens, Greece)     Volume:  26     ISSN:  1791-7549     ISO Abbreviation:  In Vivo     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806809     Medline TA:  In Vivo     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  191-5     Citation Subset:  IM    
MME, Institute of General Practice, OE5440, Hannover Medical School, Carl-Neuberg-Str.1, 30625 Hannover, Germany.
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