Document Detail


In Vivo Monitoring of Multiple Circulating Cell Populations Using Two-photon Flow Cytometry.
MedLine Citation:
PMID:  19221581     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
To detect and quantify multiple distinct populations of cells circulating simultaneously in the blood of living animals, we developed a novel optical system for two-channel, two-photon flow cytometry in vivo. We used this system to investigate the circulation dynamics in live animals of breast cancer cells with low (MCF-7) and high (MDA-MB-435) metastatic potential, showing for the first time that two different populations of circulating cells can be quantified simultaneously in the vasculature of a single live mouse. We also non-invasively monitored a population of labeled, circulating red blood cells for more than two weeks, demonstrating that this technique can also quantify the dynamics of abundant cells in the vascular system for prolonged periods of time. These data are the first in vivo application of multichannel flow cytometry utilizing two-photon excitation, which will greatly enhance our capability to study circulating cells in cancer and other disease processes.
Authors:
Eric R Tkaczyk; Cheng Frank Zhong; Jing Yong Ye; Andrzej Myc; Thommey Thomas; Zhengyi Cao; Raimon Duran-Struuck; Kathryn E Luker; Gary D Luker; Theodore B Norris; James R Baker
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Optics communications     Volume:  281     ISSN:  0030-4018     ISO Abbreviation:  -     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2010-11-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9876434     Medline TA:  Opt Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  888-894     Citation Subset:  -    
Affiliation:
Center for Ultrafast Optical Science, EECS Department, University of Michigan, 2200 Bonisteel Blvd., Ann Arbor, MI 48109-2099.
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Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
R01 CA119409-01//NCI NIH HHS; R01 CA119409-02//NCI NIH HHS; R01 CA119409-03//NCI NIH HHS; R33 CA112141-01A1//NCI NIH HHS; R33 CA112141-02//NCI NIH HHS; R33 CA112141-03//NCI NIH HHS; R01 CA119409-01//NCI NIH HHS; R01 CA119409-02//NCI NIH HHS; R01 CA119409-03//NCI NIH HHS; R33 CA112141-01A1//NCI NIH HHS; R33 CA112141-02//NCI NIH HHS; R33 CA112141-03//NCI NIH HHS

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