| An In Vivo Model of Epithelial to Mesenchymal Transition Reveals a Mitogenic Switch. | |
| | |
MedLine Citation:
|
PMID: 22906417 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The Epithelial to Mesenchymal Transition (EMT) is a process by which differentiated epithelial cells transition to a mesenchymal phenotype. EMT enables the escape of epithelial cells from the rigid structural constraints of the tissue architecture to a phenotype more amenable to cell migration and, therefore, invasion and metastasis. We characterized an in vivo model of EMT and discovered that marked changes in mitogenic signaling occurred during this process. DNA microarray analysis revealed that the expression of a number of genes varied significantly between post-EMT and pre-EMT breast cancer cells. Post-EMT cancer cells upregulated mRNA encoding c-Met and the PDGF and LPA receptors, and acquired increased responsiveness to HGF, PDGF, and LPA. This rendered the post-EMT cells responsive to the growth inhibitory effects of HGF, PDGF, and LPA receptor inhibitors/antagonists. Furthermore, post-EMT cells exhibited decreased basal Raf and Erk phosphorylation, and in comparison to pre-EMT cells, their proliferation was poorly inhibited by a MEK inhibitor. These studies suggest that therapies need to be designed to target both pre-EMT and post-EMT cancer cells and that signaling changes in post-EMT cells may allow them to take advantage of paracrine signaling from the stroma in vivo. |
| | |
Authors:
|
Stephan Jahn; Mary E Law; Patrick Corsino; Nicole N Parker; Kien Pham; Bradley J Davis; Jianrong Lu; Brian K Law |
Related Documents
:
|
21383007 - Bit-1 mediates integrin-dependent cell survival through activation of the nfkappab path... 312697 - Murine t-cell heterogeneity. 16816207 - Holdfast formation in motile swarmer cells optimizes surface attachment during caulobac... 7431297 - The free surface of mouse trophoblast in culture is non-adhesive for other cells. 20818427 - Regulation of c/ebpbeta1 by ras in mammary epithelial cells and the role of c/ebpbeta1 ... 11456417 - Stem cells in a basal bilaterian. s-phase and mitotic cells in convolutriloba longifiss... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-8-17 |
Journal Detail:
|
Title: Cancer letters Volume: - ISSN: 1872-7980 ISO Abbreviation: Cancer Lett. Publication Date: 2012 Aug |
Date Detail:
|
Created Date: 2012-8-21 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012. Published by Elsevier Ireland Ltd. |
Affiliation:
|
Department of Pharmacology and Therapeutics, University of Florida, P.O. Box 100267 1600 SW Archer Road Academic Research Building, Room R5-210 Gainesville, Florida 32610. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Lung cancer stem cells: progress and prospects.
Next Document: Identification and characterisation of STMN4 and ROBO2 gene involvement in neuroblastoma cell differ...