| In Vivo Measurement of Indole-3-acetic Acid Decarboxylation in Aging Coleus Petiole Sections. | |
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MedLine Citation:
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PMID: 16663153 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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The concentration of indoleacetic acid (IAA) in plant tissues is regulated, in part, by its rate of decarboxylation. However, the commonly used in vitro assays for IAA oxidase may not accurately reflect total in vivo decarboxylation rates. A method for measuring in vivo decarboxylation was utilized in which (14)CO(2) is collected following uptake of [1-(14)C]IAA by excised tissue sections. After a 30-minute equilibration period, the evolution of (14)CO(2) was found to follow an approximately linear course with respect to both time and tissue weight.Decarboxylation rates were measured by this method in petiole sections of the Princeton clone of Coleus blumei Benth. Both the (14)CO(2) evolved per milligram tissue and the percent of [1-(14)C]IAA uptake decarboxylated were highest in sections from the youngest petioles tested, and declined in the older tissue. Thin layer chromatography of acetonitrile extracts from the [1-(14)C]IAA-treated petioles showed a decreasing amount of free IAA and an increase at the retardation factor of indoleacetylaspartate in the older sections. The decreased decarboxylation rates in the older petioles may be attributable to a generally lower metabolic rate and increased protection of the IAA by conjugation. |
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Authors:
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T Brennan; W P Jacobs |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Plant physiology Volume: 73 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 1983 Sep |
Date Detail:
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Created Date: 2010-06-29 Completed Date: 2010-06-29 Revised Date: 2010-09-14 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 1-5 Citation Subset: - |
Affiliation:
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Department of Biology, Dickinson College, Carlisle, Pennsylvania 17013. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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