Document Detail

In vivo imaging of sentinel nodes using fluorescent silica nanoparticles in living mice.
MedLine Citation:
PMID:  19830500     Owner:  NLM     Status:  MEDLINE    
PURPOSE: We examine the feasibility of fluorescent imaging system for sentinel lymph node detection by using functionalized silica nanoparticles. MATERIALS AND METHODS: We developed a functionalized RITC-SiO(2) nanoparticles containing fluorescent dye, C(28)H(31)N(2)O(3)Cl (rhodamine B isothiocyanate) inside, and subsequently synthesized (68)Ga-NOTA-RITC-SiO(2) nanoparticles. RESULTS: At 5 min after RITC-doped silica nanoparticles injection, fluorescent signals were shown in both right axillary lymph node (ALN) and injection site of living mice. Fluorescent signals were also observed at these locations in a biodistribution study. In addition, fluorescence was detected in frozen ALN sections microscopically. The percentages of doses injected per gram of tissue of axillary and brachial lymph nodes near footpad treated with (68)Ga-NOTA-RITC-SiO(2) nanoparticles were 308.3 +/- 3.4 and 41.5 +/- 6.1, respectively. Little (68)Ga radioactivity was found in other organs. CONCLUSION: Our data provide strong evidence that functionalized silica nanoparticles has a promising potential as organic lymphatic tracer in biomedical imaging such as pre- and intraoperative surgical guidance.
Yong Hyun Jeon; Young-Hwa Kim; Kihwan Choi; Jing Yu Piao; Bo Quan; Yun-Sang Lee; Jae Min Jeong; June-Key Chung; Dong Soo Lee; Myung Chul Lee; Jaetae Lee; Doo Soo Chung; Keon Wook Kang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-15
Journal Detail:
Title:  Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging     Volume:  12     ISSN:  1860-2002     ISO Abbreviation:  Mol Imaging Biol     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-24     Completed Date:  2010-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101125610     Medline TA:  Mol Imaging Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  155-62     Citation Subset:  IM    
Department of Nuclear Medicine, Seoul National University College of Medicine, 28 Yongon-dong, Jongno-gu, Seoul, South Korea.
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MeSH Terms
Frozen Sections
Gallium / pharmacokinetics,  pharmacology
Heterocyclic Compounds / pharmacokinetics,  pharmacology
Lymph Nodes / anatomy & histology*,  drug effects*
Mice, Inbred BALB C
Microscopy, Fluorescence
Molecular Imaging / methods*
Nanoparticles / chemistry*,  ultrastructure
Rhodamines / pharmacokinetics
Silicon Dioxide / pharmacokinetics,  pharmacology*
Spectrometry, Fluorescence
Tissue Distribution / drug effects
Reg. No./Substance:
0/Heterocyclic Compounds; 0/Rhodamines; 36877-69-7/rhodamine isothiocyanate; 56491-86-2/1,4,7-triazacyclononane-N,N',N''-triacetic acid; 7440-55-3/Gallium; 7631-86-9/Silicon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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