Document Detail


In vivo imaging of intraprostatic-specific gene transcription by PET.
MedLine Citation:
PMID:  21498525     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
METHODS: The two-step transcriptional amplification (TSTA) system that amplifies the prostate-specific antigen promoter activity was used for PET imaging of the reporter gene herpes simplex virus type-1 sr39 thymidine kinase (HSV1-sr39tk). The TSTA-sr39tk system was injected directly into prostates or prostatic tumors as a replication-incompetent adenovirus (AdTSTA-sr39tk) and imaged using PET.
RESULTS: AdTSTA-sr39tk was able to image prostate-specific antigen promoter transcriptional activity by 9-(4-(18)F-fluoro-3-[hydroxymethyl]butyl)guanine PET, in both mouse and canine prostates in vivo. Ex vivo small-animal PET images, scintigraphic counts, and sr39tk expression analysis confirmed the specificity of the observed signal.
CONCLUSION: Here, by combining the TSTA-amplified signal with a protocol for tracer administration, we show that in vivo PET detection of transcriptional activity is possible in both mouse and immunocompetent canine prostates. These results suggest that imaging applications using transcription-based tumor-specific promoters should be pursued to better visualize cancer foci that escape detection by conventional biopsies.
Authors:
Frédéric Pouliot; Breanne D W Karanikolas; Mai Johnson; Makoto Sato; Saul J Priceman; David Stout; Joanne Sohn; Nagichettiar Satyamurthy; Jean B deKernion; Lily Wu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-04-15
Journal Detail:
Title:  Journal of nuclear medicine : official publication, Society of Nuclear Medicine     Volume:  52     ISSN:  1535-5667     ISO Abbreviation:  J. Nucl. Med.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-03     Completed Date:  2011-07-05     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  0217410     Medline TA:  J Nucl Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  784-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics
Animals
Dogs
Feasibility Studies
Genes, Reporter / genetics
HEK293 Cells
Herpesvirus 1, Human / enzymology
Humans
Immunocompetence / genetics
Male
Mice
Neoplasm Staging
Organ Specificity
Positron-Emission Tomography*
Promoter Regions, Genetic / genetics
Prostate / metabolism*
Prostate-Specific Antigen / genetics
Prostatic Neoplasms / genetics,  pathology
Thymidine Kinase / genetics
Transcription, Genetic*
Grant Support
ID/Acronym/Agency:
P50 CA092131/CA/NCI NIH HHS; P50 CA092131/CA/NCI NIH HHS; P50 CA092131-09/CA/NCI NIH HHS; R01 CA101904/CA/NCI NIH HHS; R01 CA101904/CA/NCI NIH HHS; R01 CA101904-07/CA/NCI NIH HHS; R01 CA101904-08/CA/NCI NIH HHS; R21 CA122693/CA/NCI NIH HHS; R21 CA122693/CA/NCI NIH HHS; R21 CA122693-02/CA/NCI NIH HHS; T32 CA009056/CA/NCI NIH HHS; //Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
EC 2.7.1.21/Thymidine Kinase; EC 3.4.21.77/Prostate-Specific Antigen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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