Document Detail

In vivo expression of Helicobacter pylori virulence genes in patients with gastritis, ulcer, and gastric cancer.
MedLine Citation:
PMID:  22124657     Owner:  NLM     Status:  MEDLINE    
The best-studied Helicobacter pylori virulence factor associated with development of peptic ulcer disease or gastric cancer (GC) rather than asymptomatic nonatrophic gastritis (NAG) is the cag pathogenicity island (cagPAI), which encodes a type IV secretion system (T4SS) that injects the CagA oncoprotein into host epithelial cells. Here we used real-time reverse transcription-PCR (RT-PCR) to measure the in vivo expression of genes on the cagPAI and of other virulence genes in patients with NAG, duodenal ulcer (DU), or GC. In vivo expression of H. pylori virulence genes was greater overall in gastric biopsy specimens of patients with GC than in those of patients with NAG or DU. However, since in vitro expression of cagA was not greater in H. pylori strains from patients with GC than in those from patients with NAG or DU, increased expression in GC in vivo is likely a result of environmental conditions in the gastric mucosa, though it may in turn cause more severe pathology. Increased expression of virulence genes in GC may represent a stress response to elevated pH or other environmental conditions in the stomach of patients with GC, which may be less hospitable to H. pylori colonization than the acidic environment in patients with NAG or DU.
Francisco Avilés-Jiménez; Adriana Reyes-Leon; Erik Nieto-Patlán; Lori M Hansen; Juan Burgueño; Irma P Ramos; Margarita Camorlinga-Ponce; Hector Bermúdez; Juan M Blancas; Lourdes Cabrera; Rosa María Ribas-Aparicio; Jay V Solnick; Javier Torres-López
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-11-28
Journal Detail:
Title:  Infection and immunity     Volume:  80     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-23     Completed Date:  2012-03-27     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  594-601     Citation Subset:  IM    
Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, UMAE Pediatría, IMSS, México City, Mexico.
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MeSH Terms
Antigens, Bacterial / genetics,  metabolism
Bacterial Proteins / genetics,  metabolism
Duodenal Ulcer / microbiology*
Gastric Mucosa / microbiology
Gastritis / microbiology*
Gene Expression Regulation, Bacterial
Helicobacter Infections / microbiology*
Helicobacter pylori / genetics*,  pathogenicity*
Stomach Neoplasms / microbiology*
Virulence / genetics
Grant Support
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Proteins; 0/cagA protein, Helicobacter pylori

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