| An In Vivo Experiment to Improve Pulmonary Absorption of Insulin Using Microbubbles. | |
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MedLine Citation:
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PMID: 21745138 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract Background: Gas-filled phospholipid-based ultrasonic microbubbles (PUMs) are widely used in diagnostic imaging. The micro- or nanoparticle size and the physiochemical nature of shell provide the potential for a new way to improve pulmonary absorption for peptides and proteins. Methods: Male Sprague-Dawley rats were fasted for 12 h. Then insulin solution and insulin-PUM mixture solution were administered by intratracheal instillation. The hypoglycemic effect was observed to evaluate insulin absorption after lung administration. Fluorescein isothiocyanate-dextran (molecular mass, 4 kDa) was used as the index of evaluating drug alveolar deposition and absorption by visualization techniques. Results: Administration of insulin solution containing PUMs significantly reduced the blood glucose levels of Sprague-Dawley rats, compared with administration of insulin-only solution. The minimum reductions of the blood glucose concentration produced by insulin solution containing PUMs and by an insulin-only solution reached 60.81% and 34.60% of the initial glucose levels, respectively, and their bioavailabilities relative to subcutaneous injection were 48.58% and 29.09%, respectively. Histopathological study of the lung showed no changes in the morphology of the pulmonary alveoli after administration to these drugs. Only a slight inflammatory cell infiltration in the alveoli could be found in some rats. Conclusion: These results suggested that PUMs might be used as an effective way to improve pulmonary absorption for peptides and proteins. |
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Authors:
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Ying-Zheng Zhao; Yan-Yan Xu; Xing Li; Cui-Tao Lu; Lu Zhang; Dan-Dan Dai; Chang-Zheng Sun; Hai-Feng Lv; Xiao-Kun Li; Wei Yang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-11 |
Journal Detail:
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Title: Diabetes technology & therapeutics Volume: - ISSN: 1557-8593 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100889084 Medline TA: Diabetes Technol Ther Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Wenzhou Medical College , Wenzhou, Zhejiang Province, China . |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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