| In vivo differentiation of magnetically labeled mesenchymal stem cells into hepatocytes for cell therapy to repair damaged liver. | |
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MedLine Citation:
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PMID: 20808237 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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OBJECTIVES: It was unclear whether systemically administered mesenchymal stem cells (MSCs) labeled with magnetic nanoparticles can transdifferentiate into hepatocytes. In the present study, we built a new in vivo murine model for monitoring the transdifferentiation of magnetically labeled green fluorescent protein (GFP) positive MSCs into albumin-positive hepatocytes, under the carbon tetrachloride (CCl4) induced persistent liver damage. We also tracked magnetically labeled MSCs by using magnetic resonance imaging (MRI) in vivo. MATERIALS AND METHODS: Among the liver damage groups, magnetically labeled GFP-positive MSCs (group A), GFP-positive MSCs (group B), and saline alone (group C) were intravenously injected. In control groups without CCl4 administration magnetically labeled GFP-positive MSCs (group D) were infused, whereas nothing was given in group E. MRI examinations were performed 24 hours and 4 weeks after cell injection in group A, B, and C. Liver-to-muscle contrast-to-noise ratios on T2*-weighted MR images were measured. At 4 weeks, 3 serum biologic liver function markers were analyzed, and mice in all groups were killed for histologic examination. RESULTS: The results showed that migration of transplanted magnetic labeled cells to the liver was successfully documented with in vivo MRI. Serum liver function markers were changed for all liver damage groups than nondamage control groups (P < 0.05), but still insignificant compared with group C (P > 0.05). Hematoxylin and eosin and Masson staining confirmed the presence of liver damage and hepatic fibrosis in group A, B, and C. Positive Prussian blue stained cells were highly correlated with GFP-positive cells in group A with an average matching rate of 95%. In group D, no iron-GFP-positive cells can be found in the liver. Albumin was expressed in (34% ± 6%) and (35% ± 7%) of GFP-positive cells in group A and B, respectively, and there was no significant difference between the 2 groups. CONCLUSIONS: Our data demonstrate that magnetic labeling technique synchronized well in GFP expressing MSCs and did not interfere with the transdifferentiation process and amending function of MSCs in vivo. Both magnetically labeled and unlabeled MSCs appeared to have the potential to differentiate into hepatocytes. |
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Authors:
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Shenghong Ju; Gao-Jun Teng; Haihua Lu; JiYang Jin; Yu Zhang; Aifeng Zhang; Yicheng Ni |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Investigative radiology Volume: 45 ISSN: 1536-0210 ISO Abbreviation: Invest Radiol Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0045377 Medline TA: Invest Radiol Country: United States |
Other Details:
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Languages: eng Pagination: 625-33 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Imaging, Department of Radiology, Zhongda Hospital, Southeast University, Nanjing, China. jsh0836@hotmail.com |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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