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In vitro wound healing improvement by low-level laser therapy application in cultured gingival fibroblasts.
MedLine Citation:
PMID:  22844284     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to determine adequate energy doses using specific parameters of LLLT to produce biostimulatory effects on human gingival fibroblast culture. Cells (3 × 10(4) cells/cm(2)) were seeded on 24-well acrylic plates using plain DMEM supplemented with 10% fetal bovine serum. After 48-hour incubation with 5% CO(2) at 37°C, cells were irradiated with a InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm; 40 mW) with energy doses of 0.5, 1.5, 3, 5, and 7 J/cm(2). Cells were irradiated every 24 h totalizing 3 applications. Twenty-four hours after the last irradiation, cell metabolism was evaluated by the MTT assay and the two most effective doses (0.5 and 3 J/cm(2)) were selected to evaluate the cell number (trypan blue assay) and the cell migration capacity (wound healing assay; transwell migration assay). Data were analyzed by the Kruskal-Wallis and Mann-Whitney nonparametric tests with statistical significance of 5%. Irradiation of the fibroblasts with 0.5 and 3 J/cm(2) resulted in significant increase in cell metabolism compared with the nonrradiated group (P < 0.05). Both energy doses promoted significant increase in the cell number as well as in cell migration (P < 0.05). These results demonstrate that, under the tested conditions, LLLT promoted biostimulation of fibroblasts in vitro.
Authors:
Fernanda G Basso; Taisa N Pansani; Ana Paula S Turrioni; Vanderlei S Bagnato; Josimeri Hebling; Carlos A de Souza Costa
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Publication Detail:
Type:  Journal Article     Date:  2012-07-15
Journal Detail:
Title:  International journal of dentistry     Volume:  2012     ISSN:  1687-8736     ISO Abbreviation:  Int J Dent     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-30     Completed Date:  2012-08-23     Revised Date:  2013-03-21    
Medline Journal Info:
Nlm Unique ID:  101524183     Medline TA:  Int J Dent     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  719452     Citation Subset:  -    
Affiliation:
Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (UNICAMP), 13414-903 Piracicaba, SP, Brazil.
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