Document Detail


In vitro toxicity assessment of amphiphillic polymer-coated CdSe/ZnS quantum dots in two human liver cell models.
MedLine Citation:
PMID:  23039050     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Semiconductor quantum dots (Qdots) are a promising new technology with benefits in the areas of medical diagnostics and therapeutics. Qdots generally consist of a semiconductor core, capping shell, and surface coating. The semiconductor core of Qdots is often composed of group II and VI metals (e.g., Cd, Se, Te, Hg) that are known to have toxic properties. Various surface coatings have been shown to stabilize Qdots and thus shield cells from the toxic properties of their core elements. In this study, HepG2 cells and primary human liver (PHL) cells were chosen as in vitro tissue culture models of human liver to examine the possible adverse effects of tri-n-octylphosphine oxide, poly(maleic anhydride-alt-1-tetradecene) copolymer (TOPO-PMAT)-coated CdSe/ZnS Qdots (TOPO-PMAT Qdots). The TOPO-PMAT coating is desirable for increasing aqueous solubility and ease of conjugation to targeting moieties (e.g., aptamers and peptides). HepG2 cells avidly incorporated these TOPO-PMAT Qdots into subcellular vesicles. However, PHL cells did not efficiently take up TOPO-PMAT Qdots, but nonparenchymal cells did (especially Kupffer cells). No acute toxicity or morphological changes were noted in either system at the exposure levels used (up to 40 nM). However, cellular stress markers and pro-inflammatory cytokines/chemokines were increased in the PHL cell cultures, suggesting that TOPO-PMAT Qdots are not likely to cause acute cytotoxicity in the liver but may elicit inflammation/hepatitis, demonstrating the importance of relevant preclinical safety models. Thus, further in vivo studies are warranted to ensure that TOPO-PMAT-coated Qdots used in biomedical applications do not induce inflammatory responses as a consequence of hepatic uptake.
Authors:
Wesley E Smith; Jessica Brownell; Collin C White; Zahra Afsharinejad; Jesse Tsai; Xiaoge Hu; Stephen J Polyak; Xiaohu Gao; Terrance J Kavanagh; David L Eaton
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-10-18
Journal Detail:
Title:  ACS nano     Volume:  6     ISSN:  1936-086X     ISO Abbreviation:  ACS Nano     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-27     Completed Date:  2013-05-16     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9475-84     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cadmium Compounds / toxicity*
Cell Line
Cell Survival / drug effects
Coated Materials, Biocompatible / toxicity*
Hepatocytes / cytology,  drug effects*,  physiology
Humans
Hydrophobic and Hydrophilic Interactions
Organophosphorus Compounds / toxicity
Polymers / toxicity*
Quantum Dots*
Selenium Compounds / toxicity*
Sulfides / toxicity*
Zinc Compounds / toxicity*
Grant Support
ID/Acronym/Agency:
P30 ES007033/ES/NIEHS NIH HHS; P30ES007033/ES/NIEHS NIH HHS; R01ES016189/ES/NIEHS NIH HHS; R21AT002895/AT/NCCAM NIH HHS; T32 ES007032/ES/NIEHS NIH HHS; T32ES007032/ES/NIEHS NIH HHS; U19 ES019545/ES/NIEHS NIH HHS; U19ES019495/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Cadmium Compounds; 0/Coated Materials, Biocompatible; 0/Organophosphorus Compounds; 0/Polymers; 0/Selenium Compounds; 0/Sulfides; 0/Zinc Compounds; 0/poly(1-tetradecene-alt-maleic acid); 78-50-2/trioctyl phosphine oxide; A7F646JC5C/cadmium selenide; KPS085631O/zinc sulfide
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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