Document Detail


In Vitro Study of Adherence, Invasion, and Persistence of Streptococcus iniae in Fibroblastic-Like Fish Cell Line SAF-1.
MedLine Citation:
PMID:  22897319     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Abstract Streptococcus iniae is a major fish pathogen producing invasive infections that result in economic losses in aquaculture. Gentamicin protection assays were used to investigate the ability of different S. iniae strains to invade and adhere to fibroblastic-like fish cell line SAF-1. All strains tested were detected intracellularly using both techniques, with variable internalization degrees between strains. The experiments carried out at 4°C demonstrated that active cell metabolism is necessary for bacterial internalization. Intracellular bacteria were detected for up to 3 d with a round morphology and were stained with 4',6-diamidino-2-phenylindole (DAPI), indicating that some bacterial cells may remain viable inside SAF-1 cells. Our in vitro findings indicate that S. iniae is capable of adhering, entering, and surviving within fibroblastic cells, which may be important for the persistence and establishment of a carrier state. Received July 8, 2011; accepted February 12, 2012.
Authors:
F El Aamri; F Real; F Acosta; B Acosta; J Valdivia; J Ramos-Vivas; D Padilla
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of aquatic animal health     Volume:  24     ISSN:  0899-7659     ISO Abbreviation:  J. Aquat. Anim. Health     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9884881     Medline TA:  J Aquat Anim Health     Country:  United States    
Other Details:
Languages:  eng     Pagination:  165-70     Citation Subset:  IM    
Affiliation:
a University Institute of Animal Health (IUSA) , University of Las Palmas de Gran Canaria , 35413 Arucas , Las Palmas , Spain.
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