Document Detail

In vitro regulation of neural differentiation and axon growth by growth factors and bioactive nanofibers.
MedLine Citation:
PMID:  20367289     Owner:  NLM     Status:  MEDLINE    
Human embryonic stem cell (ESC)-derived neural cells are a potential cell source for neural tissue regeneration. Understanding the biochemical and biophysical regulation of neural differentiation and axon growth will help us develop cell therapies and bioactive scaffolds. We demonstrated that basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) had different effects on human ESC differentiation into neural cells. EGF was more effective in inducing expression of neuron and glial markers and cell extensions. In addition to biochemical cues, poly(l-lactic acid) scaffolds with aligned nanofibers increased axon growth from ESC-derived neural cells, demonstrating the significant effects of biophysical guidance at nanoscale. To combine the biochemical and biophysical cues, bFGF and EGF were either adsorbed or bound to heparin on nanofibrous scaffolds. EGF, but not bFGF, was effectively adsorbed onto nanofibers. However, adsorbed EGF and bFGF did not effectively enhance axon growth. In contrast, immobilization of bFGF or EGF onto nanofibers using heparin as the adapter molecule significantly promoted axon growth. This study elucidated the effect of bFGF and EGF in neural differentiation and axon growth, and demonstrated a method to immobilize active bFGF and EGF onto aligned nanofibers to promote neural tissue regeneration.
Hayley J Lam; Shyam Patel; Aijun Wang; Julia Chu; Song Li
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  16     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-06     Completed Date:  2011-01-25     Revised Date:  2011-08-03    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2641-8     Citation Subset:  IM    
Department of Bioengineering, University of California at Berkeley, Berkeley, California 94720, USA.
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MeSH Terms
Axons / physiology,  ultrastructure*
Biocompatible Materials / chemistry*
Cell Differentiation
Cell Enlargement
Cell Line
Cell Proliferation
Embryonic Stem Cells / cytology*,  physiology
Nanostructures / chemistry*,  ultrastructure*
Neurons / cytology*,  physiology
Particle Size
Surface Properties
Tissue Engineering / methods*
Reg. No./Substance:
0/Biocompatible Materials

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