| In vitro proteolytic inhibition, polyphenol oxidase activity, and soluble o-diphenols in grasses and cereals. | |
| | |
MedLine Citation:
|
PMID: 20028129 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Producing high quality forage remains a challenge due to potential protein degradation during ensiling. Ten grass species and four cereals were screened for proteolytic inhibition, polyphenol oxidase (PPO) activity, and the presence of soluble PPO substrates. In orchardgrass, ryegrass, smooth bromegrass, and meadow fescue extracts, the addition of caffeic acid resulted in 70-98% reduction in proteolysis for both greenhouse and field grown environments. These same grass extracts exhibited 10- to 1000-fold higher PPO activity compared to all other grasses and cereals, depending on the substrate supplied and environment grown. Chlorogenic acid and caffeic acid were the two abundant soluble o-diphenols. Tall fescue and timothy exhibiting low PPO activity contained the highest levels of chlorogenic acid (3-11 micromol g(-1) FW): 10- to 100-fold more than any o-diphenol in all other grasses. These results suggest that several grass species contain PPO activity, but may lack appropriate o-diphenol substrates to effectively inhibit proteolysis during ensiling while other grasses lack PPO activity but contain potential soluble substrate. Environmental factors appear to influence these parameters, creating an opportunity to exploit and enhance proteolytic inhibition during ensiling by genetic manipulation. |
| | |
Authors:
|
Jane M Marita; Ronald D Hatfield; Geoffrey Brink |
Related Documents
:
|
21094199 - Modifications of the organic and mineral fractions of dental tissues following conditio... 11130179 - D-amino acid oxidase: new findings. 2852959 - Synthesis of cardiolipin derivatives with protection of the free hydroxyl: its applicat... 2408419 - Production of 6-hydroxydopa by human tyrosinase. 18985489 - Hydrocaffeic and p-coumaric acids, natural phenolic compounds, inhibit uv-b damage in w... 15383589 - Il-4-induced gene-1 is a leukocyte l-amino acid oxidase with an unusual acidic ph prefe... 1033209 - Umbilical uptake of amino acids in the unstressed fetal lamb. 8223089 - Transhepatic topical dissolution of gallbladder stones with mtbe and edta. results, sid... 10347179 - Nitrogen dioxide induces cis-trans-isomerization of arachidonic acid within cellular ph... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of agricultural and food chemistry Volume: 58 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2010 Jan |
Date Detail:
|
Created Date: 2010-01-20 Completed Date: 2010-03-25 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 959-66 Citation Subset: IM |
Affiliation:
|
US Dairy Forage Research Center, USDA-Agricultural Research Service, Madison, Wisconsin 53706, USA. J.Marita@ars.usda.gov |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Caffeic Acids
/
chemistry,
metabolism Catechol Oxidase / antagonists & inhibitors, chemistry*, metabolism Cereals / chemistry, enzymology* Chlorogenic Acid / chemistry, metabolism Hydrolysis Kinetics Plant Proteins / antagonists & inhibitors, chemistry*, metabolism Poaceae / chemistry, enzymology* Substrate Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Caffeic Acids; 0/Plant Proteins; 327-97-9/Chlorogenic Acid; 331-39-5/caffeic acid; EC 1.10.3.1/Catechol Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Development and Validation of a Liquid Chromatography Tandem Mass Spectrometry Method for Simultaneo...
Next Document: Dynamics of Ultrafast Singlet and Triplet Charge Transfer in Anthraquinone-DNA Conjugates.