| The In Vitro Protective Effects of Curcumin and Demethoxycurcumin in Curcuma longa Extract on Advanced Glycation End Products-Induced Mesangial Cell Apoptosis and Oxidative Stress. | |
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MedLine Citation:
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PMID: 22923199 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Curcuma longa L. (CLL), a traditional herbal medicine, has been widely used for the prevention of diabetic vascular complications in recent years. However, the protective effects of curcuminoids in CLL on the AGEs-induced damage to mesangial cell are not fully understood. In this present study, dihydroethidium, superoxide dismutase kit, malondialdehyde kit, and acridine orange/ethidium bromide staining methods were used to evaluate the activities of curcumin and demethoxycurcumin (10-11 - 10-9 M) on AGEs-induced oxidative stress and apoptosis, which were associated with the damage to mesangial cell. The results showed that these two compounds could significantly restore advanced glycation end products (AGEs)-induced apoptosis to normal levels (IC50 = 3.874 × 10-11 M for curcumin and IC50 = 6.085 × 10-11 M for demethoxycurcumin) and reduce remarkably reactive oxygen species generation in mesangial cell. Furthermore, curcumin and demethoxycurcumin dramatically elevated AGEs-decreased superoxide dismutase activity while significantly reducing AGEs-increased malondialdehyde content in cell culture supernatant. Our results suggest that both curcumin and demethoxycurcumin have a significant protective potential to the prevention of diabetic nephropathy. |
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Authors:
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Ji-Ping Liu; Liang Feng; Mao-Mao Zhu; Ru-Shang Wang; Ming-Hua Zhang; Shao-Ying Hu; Xiao-Bin Jia; Jin-Jie Wu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-24 |
Journal Detail:
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Title: Planta medica Volume: - ISSN: 1439-0221 ISO Abbreviation: Planta Med. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0066751 Medline TA: Planta Med Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Georg Thieme Verlag KG Stuttgart · New York. |
Affiliation:
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Department of Pharmacology, Shaanxi University of Chinese Medicine, Xianyang, P. R. China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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