| In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages. | |
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MedLine Citation:
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PMID: 22262444 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Macrophages are pivotal cells in immunity against a wide range of pathogens. Their most important property, as suggested by their name, is to ingest pathogens, leading to their killing, the release of inflammatory mediators and antigen processing. On the other hand, macrophages can also be exploited by microbes as a niche for survival in the host, as exemplified by Cryptococcus neoformans. This encapsulated yeast is an important cause of meningoencephalitis in immunocompromised people, particularly those with AIDS. Using culture and microscopy techniques, we present here methods that can be used to quantify phagocytosis of C. neoformans and its killing by macrophages, as well as the viability of the phagocyte after interaction. |
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Authors:
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André Moraes Nicola; Arturo Casadevall |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 844 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2012-01-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 189-97 Citation Subset: IM |
Affiliation:
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Departments of Microbiology and Immunology and Medicine, Albert Einstein College of Medicine of the Yeshiva University, 1300 Morris Park Avenue, Bronx, NY, USA, andre.nicola@gmail.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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