Document Detail


In vitro-generated DC with tolerogenic functions: perspectives for in vivo cellular therapy.
MedLine Citation:
PMID:  20941608     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dendritic cells (DCs) have a central role in immune regulation and serve as an essential link between innate and adaptive immunity. Their broad range of powerful immune stimulatory as well as regulatory functions has made DCs a target for vaccine development strategies. One approach to promote the tolerogenicity of DCs is to suppress their maturation by pharmacological agents, including the glucocorticoid dexamethasone. In this chapter, we describe methods to generate tolerogenic Dex-DC derived from either human peripheral blood monocytes or rat bone marrow cells.
Authors:
Cees van Kooten; Kyra A Gelderman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  677     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2010-10-13     Completed Date:  2011-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  149-59     Citation Subset:  IM    
Affiliation:
Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands. kooten@lumc.nl
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow Cells / drug effects*,  immunology
Cell Differentiation / physiology
Cells, Cultured
Cytokines / pharmacology
Dendritic Cells / drug effects*,  immunology
Dexamethasone / pharmacology
Humans
Immune Tolerance / drug effects*
Immunosuppressive Agents / pharmacology
Internationality
Organic Chemicals / toxicity
Rats
Tissue Therapy / utilization*
Chemical
Reg. No./Substance:
0/Cytokines; 0/Immunosuppressive Agents; 0/Organic Chemicals; 0/Tolerogen; 50-02-2/Dexamethasone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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