Document Detail

In Vitro Evaluation of Silver Nanoparticles on Human Tumoral and Normal Cells.
MedLine Citation:
PMID:  23278213     Owner:  NLM     Status:  Publisher    
Abstract Silver nanoparticles (AgNPs), which have well-known antimicrobial properties, are extensively used in various medical and general applications. Despite the widespread use of AgNPs, relatively few studies have been undertaken to determine the toxicity effects of AgNPs exposure. The aim of the present work was to study how AgNPs interact with four different human cell lines (hepatoma, leukemia, dermal and pulmonary fibroblast) in order to understand the impact of such nanomaterials on cellular biological functions. For toxicity evaluations, mitochondrial function (MTT assay) and membrane leakage of lactate dehydrogenase (LDH assay) were assessed under control and exposed conditions (24, 48 and 72 h of exposure). Furthermore we evaluated the protective effect of N-Acetyl-L-cysteine (NAC) against AgNPs-induced cytotoxicity. Results showed that mitochondrial function decreased in all cell lines exposed to AgNPs at 6.72-13.45 µg/ml. LDH leakage also increased in all cell lines exposed to AgNPs (6.72-13.45 µg/ml). However, the cytotoxic effect of AgNPs (13.45 µg/ml) was prevented by pretreatment of different concentrations of NAC (1-20 mM). Our findings indicate that AgNPs are cytotoxic on human tumor and normal cells, being the tumor cells more sensitive to the cytotoxic effect of AgNPs. In addition, NAC protects human cells from cytotoxicity of silver nanoparticles, suggesting that oxidative stress is in part responsible of this effect.
Alicia Avalos; Ana Isabel Haza; Diego Mateo; Paloma Morales
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-2
Journal Detail:
Title:  Toxicology mechanisms and methods     Volume:  -     ISSN:  1537-6524     ISO Abbreviation:  Toxicol. Mech. Methods     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134521     Medline TA:  Toxicol Mech Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos. Facultad de Veterinaria. Universidad Complutense de Madrid. 28040 Madrid (Spain).
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