| In vitro communities derived from oral and gut microbial floras inhibit the growth of bacteria of foreign origins. | |
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MedLine Citation:
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PMID: 20625712 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The gastrointestinal (GI) tract is home to trillions of microbes. Within the same GI tract, substantial differences in the bacterial species that inhabit the oral cavity and intestinal tract have been noted. While the influence of host environments and nutritional availability in shaping different microbial communities is widely accepted, we hypothesize that the existing microbial flora also plays a role in selecting the bacterial species that are being integrated into the community. In this study, we used cultivable microbial communities isolated from different parts of the GI tract of mice (oral cavity and intestines) as a model system to examine this hypothesis. Microbes from these two areas were harvested and cultured using the same nutritional conditions, which led to two distinct microbial communities, each with about 20 different species as revealed by PCR-based denaturing gradient gel electrophoresis analysis. In vitro community competition assays showed that the two microbial floras exhibited antagonistic interactions toward each other. More interestingly, all the original isolates tested and their closely related species displayed striking community preferences: They persisted when introduced into the bacterial community of the same origin, while their viable count declined more than three orders of magnitude after 4 days of coincubation with the microbial flora of foreign origin. These results suggest that an existing microbial community might impose a selective pressure on incoming foreign bacterial species independent of host selection. The observed inter-flora interactions could contribute to the protective effect of established microbial communities against the integration of foreign bacteria to maintain the stability of the existing communities. |
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Authors:
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Xuesong He; Yan Tian; Lihong Guo; Takashi Ano; Renate Lux; David R Zusman; Wenyuan Shi |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-07-13 |
Journal Detail:
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Title: Microbial ecology Volume: 60 ISSN: 1432-184X ISO Abbreviation: Microb. Ecol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-14 Completed Date: 2011-01-19 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 7500663 Medline TA: Microb Ecol Country: United States |
Other Details:
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Languages: eng Pagination: 665-76 Citation Subset: IM |
Affiliation:
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UCLA School of Dentistry, 10833 Le Conte Avenue, CHS 20-114, Los Angeles, CA 90095-1668, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibiosis* Bacteria / growth & development* DNA, Bacterial / genetics Denaturing Gradient Gel Electrophoresis Gastrointestinal Tract / microbiology* Male Mice Mice, Inbred C57BL Mouth / microbiology* Sequence Analysis, DNA |
| Grant Support | |
ID/Acronym/Agency:
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GM54666/GM/NIGMS NIH HHS; R01 GM054666-13/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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