Document Detail

In vitro bactericidal effects of 625, 525, and 425 nm wavelength (red, green, and blue) light-emitting diode irradiation.
MedLine Citation:
PMID:  24138193     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: The purpose of this study was to evaluate the relationship of 625, 525, and 425 nm wavelengths, providing average power output and effects on three common pathogenic bacteria.
BACKGROUND DATA: Ultraviolet (UV) light kills bacteria, but the bactericidal effects of UV may not be unique, as 425 nm produces a similar effect. The bactericidal effects of light-emitting diode (LED) wavelengths such as 625 and 525 nm have not been described. Before conducting clinical trials, the appropriate wavelength with reasonable dose and exposure time should be established.
MATERIALS AND METHODS: The bactericidal effects of 625, 525, and 425 nm wavelength LED irradiation were investigated in vitro for the anaerobic bacterium Porphyromonas gingivalis and two aerobes (Staphylococcus aureus and Escherichia coli DH5α). Average power output was 6 mW/cm(2) for 1 h. The bacteria were exposed to LED irradiation for 1, 2, 4, and 8 h (21.6, 43.2, 86.4, and 172.8 J/cm(2), respectively). LED irradiation was performed during growth on agar and in broth. Control bacteria were incubated without LED irradiation. Bacterial growth was expressed in colony-forming units (CFU) and at an optical density at 600 nm in agar and broth.
RESULTS: The bactericidal effect of LED phototherapy depended upon wavelength, power density, bacterial viable number, and bacteria species. The bactericidal effect of 425 and 525 nm irradiation varied depending upon the bacterial inoculation, compared with unirradiated samples and samples irradiated with red light. Especially, P. gingivalis and E. coli DH5α were killed by 425 nm, and S. aureus growth was inhibited by 525 nm. However, the wavelength of 625 nm was not bactericidal for P. gingivalis, E. coli DH5α, or S. aureus.
CONCLUSIONS: Irradiation at 625 nm light was not bactericidal to S. aureus, E. coli, and P. gingivalis, whereas wavelengths of 425 and 525 nm had bactericidal effects. S. aureus was also killed at 525 nm.
SangWoo Kim; Jisun Kim; WonBong Lim; SangMi Jeon; OkSu Kim; Jeong-Tae Koh; Chang-Su Kim; HongRan Choi; OkJoon Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-10-19
Journal Detail:
Title:  Photomedicine and laser surgery     Volume:  31     ISSN:  1557-8550     ISO Abbreviation:  Photomed Laser Surg     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-11-04     Completed Date:  2014-10-22     Revised Date:  2014-11-04    
Medline Journal Info:
Nlm Unique ID:  101222340     Medline TA:  Photomed Laser Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  554-62     Citation Subset:  IM    
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MeSH Terms
Colony Count, Microbial
Dose-Response Relationship, Radiation
Escherichia coli / radiation effects*
Phototherapy / instrumentation,  methods*
Porphyromonas gingivalis / radiation effects*
Staphylococcus aureus / radiation effects*

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