| In Vitro Assessment of Factors Affecting the Apparent Diffusion Coefficient of Ramos Cells Using Bio-phantoms. | |
| | |
MedLine Citation:
|
PMID: 22729107 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkittｾs lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in vitro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values. |
| | |
Authors:
|
Takanori Sasaki; Masahiro Kuroda; Kazunori Katashima; Masakazu Ashida; Hidenobu Matsuzaki; Junichi Asaumi; Jun Murakami; Seiichiro Ohno; Hirokazu Kato; Susumu Kanazawa |
Related Documents
:
|
306847 - Clonal origin of a t cell lymphoproliferative malignancy. 14696147 - Microglandular hyperplasia has a cytomorphological spectrum overlapping with atypical s... 22367497 - Zinc induces caspase-dependent mitochondrial pathway of the programmed cell death in ha... 22472287 - Biotin, niacin, and pantothenic acid assay using lyophilized lactobacillus plantarum at... 8969267 - Protection of human nasal respiratory epithelium from complement-mediated lysis by cell... 19323627 - Expression of multidrug resistance associated protein 5 (mrp5) on cornea and its role i... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Acta medica Okayama Volume: 66 ISSN: 0386-300X ISO Abbreviation: Acta Med. Okayama Publication Date: 2012 Jun |
Date Detail:
|
Created Date: 2012-06-25 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0417611 Medline TA: Acta Med Okayama Country: Japan |
Other Details:
|
Languages: eng Pagination: 263-70 Citation Subset: IM |
Affiliation:
|
Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clostridium botulinum Type E Toxins Bind to Caco-2 Cells by a Different Mechanism from That of Type ...
Next Document: In vitro chemosensitivity using the histoculture drug response assay in human epithelial ovarian can...