Document Detail


In vitro assessment of factors affecting the apparent diffusion coefficient of Ramos cells using bio-phantoms.
MedLine Citation:
PMID:  22729107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkitt's lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in vitro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values.
Authors:
Takanori Sasaki; Masahiro Kuroda; Kazunori Katashima; Masakazu Ashida; Hidenobu Matsuzaki; Junichi Asaumi; Jun Murakami; Seiichiro Ohno; Hirokazu Kato; Susumu Kanazawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta medica Okayama     Volume:  66     ISSN:  0386-300X     ISO Abbreviation:  Acta Med. Okayama     Publication Date:  2012  
Date Detail:
Created Date:  2012-06-25     Completed Date:  2012-10-29     Revised Date:  2014-05-23    
Medline Journal Info:
Nlm Unique ID:  0417611     Medline TA:  Acta Med Okayama     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  263-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal, Murine-Derived / pharmacology
Apoptosis / drug effects
Burkitt Lymphoma / pathology*
Cell Count
Cell Line, Tumor
Diffusion
Diffusion Magnetic Resonance Imaging*
Flow Cytometry
Humans
Polysaccharides, Bacterial
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal, Murine-Derived; 0/Polysaccharides, Bacterial; 0/rituximab; 71010-52-1/gellan gum

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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