Document Detail


In vitro assessment of antibacterial activity and cytocompatibility of silver-containing PHBV nanofibrous scaffolds for tissue engineering.
MedLine Citation:
PMID:  20415469     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infections with bacteria have become a serious problem in joint arthroplasty. This study reports about in vitro antibacterial activity and in vitro cell compatibility of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers loaded with metallic silver particles of a size of 5-13 nm. In vitro antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae was studied by microplate proliferation tests. The adhesion, viability, and proliferation properties of fibroblasts (NIH 3T3) and differentiation of osteoblasts (MC3T3-E1) were done to study in vitro cell compatibility of the scaffolds. As the results, only silver-containing PHBV nanofibrous scaffolds showed a high antibacterial activity and an inhibitory effect on the growth of both Staphylococcus aureus and Klebsiella pneumoniae bacteria. The nanofibrous scaffolds having silver nanoparticles <1.0% were free of in vitro cytotoxicity. To sum up, the PHBV nanofibrous scaffolds having nanoparticles <1.0 wt % showed not only good antibacterial activity but also good in vitro cell compatibility. It is considered that the PHBV nanofibrous scaffolds with silver nanoparticles <1.0 wt % have a potential to be used in joint arthroplasty.
Authors:
Zhi-Cai Xing; Won-Pyo Chae; Jin-Young Baek; Moon-Jeong Choi; Yongsoo Jung; Inn-Kyu Kang
Related Documents :
10606029 - Chondrocyte-biocompatibility of degrapol-foam: in vitro evaluations.
19868679 - A pure strain of cartilage cells in vitro.
19756969 - Structural properties of polysaccharide-based microcapsules for soft tissue regeneration.
19780649 - Acellular cardiac extracellular matrix as a scaffold for tissue engineering: in vitro c...
12440929 - The role of disc cell heterogeneity in determining disc biochemistry: a speculation.
1800449 - Numerical evaluation of the invasion of closely related cell lines into collagen type i...
7078939 - Biochemical and morphological study of adriamycin-induced changes in murine neuroblasto...
21338339 - Active participation of cell death in development and organismal homeostasis.
7964269 - The effects of pyroglutamylglutamylprolineamide, a peptide related to thyrotrophin-rele...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomacromolecules     Volume:  11     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-10     Completed Date:  2010-08-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1248-53     Citation Subset:  IM    
Affiliation:
Department of Polymer Science and Engineering, Kyungpook National University, Daegu 702-701, Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / pharmacology*
Biocompatible Materials*
Klebsiella pneumoniae / drug effects
Mice
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
NIH 3T3 Cells
Nanostructures*
Silver / chemistry*
Staphylococcus aureus / drug effects
Tissue Engineering*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Biocompatible Materials; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Nanoscale Electrodes by Conducting Atomic Force Microscopy: Oxygen Reduction Kinetics at the Ptmid R...
Next Document:  Design of Renewable Hydrogel Release Systems from Fiberboard Mill Wastewater.