Document Detail

In vitro assessment of antibacterial activity and cytocompatibility of silver-containing PHBV nanofibrous scaffolds for tissue engineering.
MedLine Citation:
PMID:  20415469     Owner:  NLM     Status:  MEDLINE    
Infections with bacteria have become a serious problem in joint arthroplasty. This study reports about in vitro antibacterial activity and in vitro cell compatibility of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers loaded with metallic silver particles of a size of 5-13 nm. In vitro antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae was studied by microplate proliferation tests. The adhesion, viability, and proliferation properties of fibroblasts (NIH 3T3) and differentiation of osteoblasts (MC3T3-E1) were done to study in vitro cell compatibility of the scaffolds. As the results, only silver-containing PHBV nanofibrous scaffolds showed a high antibacterial activity and an inhibitory effect on the growth of both Staphylococcus aureus and Klebsiella pneumoniae bacteria. The nanofibrous scaffolds having silver nanoparticles <1.0% were free of in vitro cytotoxicity. To sum up, the PHBV nanofibrous scaffolds having nanoparticles <1.0 wt % showed not only good antibacterial activity but also good in vitro cell compatibility. It is considered that the PHBV nanofibrous scaffolds with silver nanoparticles <1.0 wt % have a potential to be used in joint arthroplasty.
Zhi-Cai Xing; Won-Pyo Chae; Jin-Young Baek; Moon-Jeong Choi; Yongsoo Jung; Inn-Kyu Kang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomacromolecules     Volume:  11     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-10     Completed Date:  2010-08-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1248-53     Citation Subset:  IM    
Department of Polymer Science and Engineering, Kyungpook National University, Daegu 702-701, Korea.
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MeSH Terms
Anti-Bacterial Agents / pharmacology*
Biocompatible Materials*
Klebsiella pneumoniae / drug effects
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
NIH 3T3 Cells
Silver / chemistry*
Staphylococcus aureus / drug effects
Tissue Engineering*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Biocompatible Materials; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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