Document Detail

In vitro anti-HIV-1 activity of the aqueous extract of Asterina pectinifera.
MedLine Citation:
PMID:  21361863     Owner:  NLM     Status:  MEDLINE    
An aqueous extract of starfish, Asterina pectinifera, was investigated for its anti-HIV-1 efficiency in vitro on human T-cell lines. A. pectinifera significantly maintained the viability of HIV-infected cells as much as 86% of the untreated infected control cells at the non-toxic concentrations (0.05~4 mg/mL) in CEM-SS cells. Anti-HIV-1 activity of A. pectinifera extract was further supported by quantification of syncytia formation, reverse transcriptase activity and Western blot analysis in C8166, CEM-SS and H9 cells, respectively. Current results demonstrated a notable inhibition of HIV-1 induced syncytia formation and HIV-1 reverse transcriptase activity assay with EC50 of 0.71 mg/mL and 0.65 mg/mL, respectively. Moreover, A. pectinifera extract treatment decreased the production of p24 protein and gene expression of HIV-1 viral infection factor in a dose-dependent manner according to immunoblot and reverse transcription polymerase chain reaction analysis. In the light of current study, it can be concluded that A. pectinifera contains highly potential anti-HIV-1 components and a further investigation for active compound isolation is urged.
Fatih Karadeniz; Mustafa Zafer Karagozlu; Chang-Suk Kong; Se-Kwon Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current HIV research     Volume:  9     ISSN:  1873-4251     ISO Abbreviation:  Curr. HIV Res.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-04-12     Completed Date:  2011-07-25     Revised Date:  2012-02-10    
Medline Journal Info:
Nlm Unique ID:  101156990     Medline TA:  Curr HIV Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  95-102     Citation Subset:  IM    
Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
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MeSH Terms
Anti-HIV Agents / isolation & purification,  pharmacology*
Asterina / chemistry*
Cell Extracts / isolation & purification,  pharmacology*
Cell Line
Cell Survival
Giant Cells / drug effects
HIV Core Protein p24 / biosynthesis
HIV-1 / drug effects*
Microbial Sensitivity Tests
T-Lymphocytes / virology
Reg. No./Substance:
0/Anti-HIV Agents; 0/Cell Extracts; 0/HIV Core Protein p24

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