| In Vitro Adherence Patterns of Shigella Serogroups to Bovine Recto-Anal Junction Squamous Epithelial (RSE) Cells Are Similar to Those of Escherichia coli O157. | |
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MedLine Citation:
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PMID: 22401271 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract The aims of this study were to determine whether Shigella species, which are human gastrointestinal pathogens, can adhere to cattle recto-anal junction squamous epithelial (RSE) cells using a recently standardized in vitro adherence assay, and to compare their adherence patterns with that of Escherichia coli O157. Shigella dysenteriae (serogroup A), S. flexneri (serogroup B), S. boydii (serogroup C), and S. sonnei (serogroup D) were tested in adherence assays using both RSE and HEp-2 cells, in the presence or absence of D+mannose. Escherichia coli O157, which adheres to RSE cells in a Type I fimbriae-independent manner, was used as a positive control. Shigella serogroups A, B, D, but not C adhered to RSE cells with distinct adherence patterns in the presence of D+mannose. No such distinction could be made between the four Shigella serogroups based on the HEp-2 cell adherence patterns. Thus, this study provides evidence that certain Shigella serogroups adhere to RSE cells in a manner that is similar to the adherence pattern of E. coli O157. These unexpected observations of in vitro binding of these foodborne human pathogens to cells of the bovine gastrointestinal tract warrant evaluation of Shigella carriage by cattle using both experimental and observational studies, especially for serogroups B and D. Such studies are currently underway. |
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Authors:
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Indira T Kudva |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-8 |
Journal Detail:
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Title: Foodborne pathogens and disease Volume: - ISSN: 1556-7125 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101120121 Medline TA: Foodborne Pathog Dis Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center, Agricultural Research Service , U.S. Department of Agriculture, Ames, Iowa. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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