Document Detail

In vitro activity of Paclitaxel-loaded polymeric expansile nanoparticles in breast cancer cells.
MedLine Citation:
PMID:  23617223     Owner:  NLM     Status:  MEDLINE    
Through a series of in vitro studies, the essential steps for intracellular drug delivery of paclitaxel using a pH-responsive nanoparticle system have been investigated in breast cancer cells. We successfully encapsulated paclitaxel within polymeric expansile nanoparticles (Pax-eNPs) at 5% loading via a miniemulsion polymerization procedure. Fluorescently tagged eNPs were readily taken up by MDA-MB-231 breast cancer cells grown in culture as confirmed by confocal microscopy and flow cytometry. The ability of the encapsulated paclitaxel to reach the cytoplasm was also observed using confocal microscopy and fluorescently labeled paclitaxel. Pax-eNPs were shown to be efficacious against three in vitro human breast adenocarcinoma cell lines (MDA-MB-231, MCF-7, and SK-BR-3) as well as cells isolated from the pleural effusions of two different breast cancer patients. Lastly, macropinocytosis was identified as the major cellular pathway responsible for eNP uptake, as confirmed using temperature-sensitive metabolic reduction, pharmacologic inhibitors, and fluid-phase marker colocalization.
Kimberly Ann V Zubris; Rong Liu; Aaron Colby; Morgan D Schulz; Yolonda L Colson; Mark W Grinstaff
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-05-09
Journal Detail:
Title:  Biomacromolecules     Volume:  14     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-10     Completed Date:  2013-11-05     Revised Date:  2014-06-11    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2074-82     Citation Subset:  IM    
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MeSH Terms
Antineoplastic Agents, Phytogenic / administration & dosage*
Breast Neoplasms / pathology*
Paclitaxel / administration & dosage*
Polymers / administration & dosage*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Polymers; 33069-62-4/Paclitaxel

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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