Document Detail


In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.
MedLine Citation:
PMID:  23055266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC = 7-hydroxycoumarin, NBD = 7-nitrobenzofurazan, TAMRA = carboxytetramethylrhodamine.
Authors:
Erkin Kuru; H Velocity Hughes; Pamela J Brown; Edward Hall; Srinivas Tekkam; Felipe Cava; Miguel A de Pedro; Yves V Brun; Michael S VanNieuwenhze
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-10
Journal Detail:
Title:  Angewandte Chemie (International ed. in English)     Volume:  51     ISSN:  1521-3773     ISO Abbreviation:  Angew. Chem. Int. Ed. Engl.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-04     Completed Date:  2013-05-31     Revised Date:  2013-12-09    
Medline Journal Info:
Nlm Unique ID:  0370543     Medline TA:  Angew Chem Int Ed Engl     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  12519-23     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Descriptor/Qualifier:
Agrobacterium tumefaciens / metabolism
Amino Acids / chemistry*
Bacillus subtilis / metabolism
Bacteria / metabolism*
Benzoxazoles / chemistry
Biosensing Techniques
Cell Wall / chemistry,  metabolism
Coumarins / chemistry
Escherichia coli / metabolism
Fluorescent Dyes / chemistry*
Microscopy
Peptidoglycan / biosynthesis*,  chemistry
Grant Support
ID/Acronym/Agency:
AI 059327/AI/NIAID NIH HHS; AI072992/AI/NIAID NIH HHS; GM051986/GM/NIGMS NIH HHS; R01 GM051986/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Benzoxazoles; 0/Coumarins; 0/Fluorescent Dyes; 0/Peptidoglycan; 273-09-6/benzofurazan; A4VZ22K1WT/coumarin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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