| In situ fluorescence visualization of transcription sites and genomic Loci in blood stages of Plasmodium falciparum. | |
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MedLine Citation:
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PMID: 22990790 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescence-based techniques have been used extensively in the malaria field to study the functional role of nuclear organization and gene positioning in blood stages of the human malaria parasite, Plasmodium falciparum. In this chapter, we present optimized protocols for bromouridine (BrUTP) incorporation into nascent RNA in live parasites and fluorescent in situ hybridization (FISH) in fixed parasites. Methodology to perform various combinations of the FISH assay, as well as a basic approach for quantitative analysis of nuclear position, is also described. |
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Authors:
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Liliana Mancio-Silva; Artur Scherf |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 923 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-09-19 Completed Date: 2013-01-30 Revised Date: 2013-02-08 |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 335-51 Citation Subset: IM |
Affiliation:
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Unité de Biologie des Interactions Hôte-Parasite, Institut Pasteur, Paris, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Erythrocytes / parasitology* Genetic Loci* Genome, Protozoan* Humans Image Processing, Computer-Assisted / methods In Situ Hybridization, Fluorescence / methods* Life Cycle Stages Plasmodium falciparum / genetics*, growth & development Transcription Initiation Site* |
| Grant Support | |
ID/Acronym/Agency:
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250320//European Research Council |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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