Document Detail


In planta expression screens of Phytophthora infestans RXLR effectors reveal diverse phenotypes, including activation of the Solanum bulbocastanum disease resistance protein Rpi-blb2.
MedLine Citation:
PMID:  19794118     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Irish potato famine pathogen Phytophthora infestans is predicted to secrete hundreds of effector proteins. To address the challenge of assigning biological functions to computationally predicted effector genes, we combined allele mining with high-throughput in planta expression. We developed a library of 62 infection-ready P. infestans RXLR effector clones, obtained using primer pairs corresponding to 32 genes and assigned activities to several of these genes. This approach revealed that 16 of the 62 examined effectors cause phenotypes when expressed inside plant cells. Besides the well-studied AVR3a effector, two additional effectors, PexRD8 and PexRD36(45-1), suppressed the hypersensitive cell death triggered by the elicitin INF1, another secreted protein of P. infestans. One effector, PexRD2, promoted cell death in Nicotiana benthamiana and other solanaceous plants. Finally, two families of effectors induced hypersensitive cell death specifically in the presence of the Solanum bulbocastanum late blight resistance genes Rpi-blb1 and Rpi-blb2, thereby exhibiting the activities expected for Avrblb1 and Avrblb2. The AVRblb2 family was then studied in more detail and found to be highly variable and under diversifying selection in P. infestans. Structure-function experiments indicated that a 34-amino acid region in the C-terminal half of AVRblb2 is sufficient for triggering Rpi-blb2 hypersensitivity and that a single positively selected AVRblb2 residue is critical for recognition by Rpi-blb2.
Authors:
Sang-Keun Oh; Carolyn Young; Minkyoung Lee; Ricardo Oliva; Tolga O Bozkurt; Liliana M Cano; Joe Win; Jorunn I B Bos; Hsin-Yin Liu; Mireille van Damme; William Morgan; Doil Choi; Edwin A G Van der Vossen; Vivianne G A A Vleeshouwers; Sophien Kamoun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-09-30
Journal Detail:
Title:  The Plant cell     Volume:  21     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-10-30     Completed Date:  2010-01-12     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2928-47     Citation Subset:  IM    
Affiliation:
Department of Plant Pathology, Ohio State University-Ohio Agricultural Research and Development Center, Wooster, Ohio 44691, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AATU01000000;  GQ869389;  GQ869390;  GQ869391;  GQ869392;  GQ869393;  GQ869394;  GQ869395;  GQ869396;  GQ869397;  GQ869398;  GQ869399;  GQ869400;  GQ869401;  GQ869402;  GQ869403;  GQ869404;  GQ869405;  GQ869406;  GQ869407;  GQ869408;  GQ869409;  GQ869410;  GQ869411;  GQ869412;  GQ869413;  GQ869414;  GQ869415;  GQ869416;  GQ869417;  GQ869418;  GQ869419;  GQ869420;  GQ869421;  GQ869422;  GQ869423;  GQ869424;  GQ869425;  GQ869426;  GQ869427;  GQ869428;  GQ869429;  GQ869430;  GQ869431;  GQ869432;  GQ869433;  GQ869434;  GQ869435;  GQ869436;  GQ869437;  GQ869438;  GQ869439;  GQ869440;  GQ869441;  GQ869442;  GQ869443;  GQ869444;  GQ869445;  GQ869446;  GQ869447;  GQ869448;  GQ869449;  GQ869450;  GQ869451;  GQ869452;  GQ869453;  GQ869454;  GQ869455;  GQ869456;  GQ869457;  GQ869458;  GQ869459;  GQ869460;  GQ869461;  GQ869462;  GQ869463;  GQ869464;  GQ869465;  GQ869466;  GQ869467;  GQ869468;  GQ869469;  GQ869470;  GQ869471;  GQ869472;  GQ869473;  GQ869474
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MeSH Terms
Descriptor/Qualifier:
Algal Proteins / metabolism*
Alleles
Amino Acid Sequence
Cell Death
Gene Expression Profiling
Gene Expression Regulation, Plant
Genes, Plant
Immunity, Innate
Molecular Sequence Data
Phytophthora infestans / metabolism,  pathogenicity*
Plant Proteins / genetics,  metabolism*
Polymorphism, Genetic
RNA, Plant / metabolism
Sequence Alignment
Sequence Analysis, DNA
Solanum / genetics*,  immunology,  metabolism
Tobacco / genetics,  immunology,  metabolism
Chemical
Reg. No./Substance:
0/Algal Proteins; 0/Plant Proteins; 0/RNA, Plant
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