Document Detail

In CK2 inactivated cells the cyclin dependent kinase inhibitor Sic1 is involved in cell-cycle arrest before the onset of S phase.
MedLine Citation:
PMID:  17574209     Owner:  NLM     Status:  MEDLINE    
Protein kinase CK2 is a heterotetramer composed of two catalytic and two regulatory subunits. In Saccharomyces cerevisiae the catalytic subunits (alpha and alpha') are encoded by the CKA1, CKA2 genes. cka1Deltacka2(ts) mutants arrest cell cycle in both G1 and G2/M at 37 degrees C. Hence, it has been proposed that CK2 plays an important role in cell-cycle progression and several cell-cycle proteins have been reported to be CK2 substrates. We have previously shown that Sic1, the inhibitor of Clb5-Cdc28 complexes required for the G1/S transition, is a physiologically relevant CK2 substrate. Here we show that CK2 inactivation up-regulates Sic1 level resulting in severe down-regulation of Clb5-Cdc28 kinase activity. Concurrent inactivation of Sic1 and CK2 leads to accumulation of cells with a post-synthetic DNA content and short/elongated spindles, typical of cells arrested in mitosis. These findings indicate that Sic1 plays a major role during G1 arrest of CK2-inactivated cells.
Farida Tripodi; Vittoria Zinzalla; Marco Vanoni; Lilia Alberghina; Paola Coccetti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-04
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  359     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-06-27     Completed Date:  2007-08-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  921-7     Citation Subset:  IM    
Dipartimento di Biotecnologie e Bioscienze, Università Milano-Bicocca, P.zza della Scienza 2, 20126 Milano, Italy.
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MeSH Terms
Apoptosis / physiology*
Casein Kinase II / metabolism*
Cell Cycle Proteins / metabolism*
Cyclin-Dependent Kinase Inhibitor Proteins
Gene Expression Regulation, Fungal / physiology
S Phase / physiology*
Saccharomyces cerevisiae / cytology*,  metabolism*
Saccharomyces cerevisiae Proteins / metabolism*
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Cyclin-Dependent Kinase Inhibitor Proteins; 0/SIC1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; EC Kinase II

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