| Improving the procedure for detection of intrahepatic transplanted islets by magnetic resonance imaging. | |
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MedLine Citation:
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PMID: 19681816 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Islet transplantation is an effective therapy for restoring normoglycemia in type-1 diabetes, but long-term islet graft function is achieved only in a minority of cases. Noninvasive magnetic resonance imaging of pancreatic islets is an attractive option for "real-time" monitoring of graft evolution. So far, previous studies have been performed in the absence of a standardized labeling procedure and, besides a feasibility study in patients, the effectiveness and safety of various labeling approaches were tested only with high field magnets (4.7 T). In this study, we addressed: (a) standardization of a labeling procedure for human islets with clinically-approved contrast agent Endorem, (b) safety aspects of labeling related to inflammation and (c) quality of imaging both at 7 T and 1.5 T. We have highlighted that the ratio of Endorem/islet is crucial for reproducible labeling, with a ratio of 2.24 ug/IEQ, allowing successful in vivo imaging both with 1.5 T and 7.0 T magnets up to 143 days after intrahepatic transplant. With this standardized labeling procedure, labeled islets are neither inflamed nor more susceptible to inflammatory insults than unlabeled ones. This report represents an important contribution towards the development of a standardized and safe clinical protocol for the noninvasive imaging of transplanted islets in humans. |
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Authors:
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M L Malosio; A Esposito; A Poletti; S Chiaretti; L Piemonti; R Melzi; R Nano; F Tedoldi; T Canu; P Santambrogio; C Brigatti; F De Cobelli; P Maffi; A Secchi; A Del Maschio |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-14 |
Journal Detail:
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Title: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons Volume: 9 ISSN: 1600-6143 ISO Abbreviation: Am. J. Transplant. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-21 Completed Date: 2009-11-30 Revised Date: 2011-11-24 |
Medline Journal Info:
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Nlm Unique ID: 100968638 Medline TA: Am J Transplant Country: United States |
Other Details:
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Languages: eng Pagination: 2372-82 Citation Subset: IM |
Affiliation:
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HSR-DRI, Università Vita-Salute San Raffaele, Milano, Italy. malosio.marialuisa@hsr.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Contrast Media Diabetes Mellitus, Type 1 / physiopathology, surgery* Feasibility Studies Gene Expression Profiling Immunohistochemistry Islets of Langerhans Transplantation* Liver / pathology* Magnetic Resonance Imaging / methods* Male Mice Mice, Inbred C57BL Reproducibility of Results |
| Grant Support | |
ID/Acronym/Agency:
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JT01Y01//Telethon |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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