Document Detail

Improving the nutritive value of tubers: elevation of cysteine and glutathione contents in the potato cultivar White Lady by marker-free transformation.
MedLine Citation:
PMID:  17116342     Owner:  NLM     Status:  MEDLINE    
The amino acids that limit the nutritive value of potato are the sulfur containing amino acids methionine and cysteine. Manipulation of the targeted amino acid biosynthesis is a way to circumvent this problem. Cysteine is synthesised from O-acetyl-l-serine formed by serine acetyltransferase (SAT). To increase the cysteine content of the commercial potato cultivar White Lady the chimeric SAT-coding cysE gene from Escherichia coli under the control of the constitutive CaMV 35S promoter and fused to the chloroplast targeting rbcS 5'-transit peptide sequence was introduced into the White Lady genome. Novelty of the approach was the application of marker-free transformation. Two transgenic lines were obtained that accumulated the cysE mRNA in high amounts. Crude leaf extracts of these plants exhibited up to 80- and 20-fold higher SAT activity in leaves and tubers, respectively, than those prepared from non-transformed plants. Levels of cysteine and glutathione both in leaves and tubers were 1.5-fold higher in average than in control plants. The alterations observed had no effect on tuber yield and sprouting behaviour. Gas chromatography coupled to mass spectrometry showed that all other amino acids than cysteine were unaffected. Here we demonstrate for the first time that the cysteine content of tubers can be enhanced by metabolic engineering.
Ibolya Stiller; Gábor Dancs; Holger Hesse; Rainer Hoefgen; Zsófia Bánfalvi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-27
Journal Detail:
Title:  Journal of biotechnology     Volume:  128     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-16     Completed Date:  2007-04-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  335-43     Citation Subset:  IM    
Agricultural Biotechnology Center, H-2101 Gödöllo, Hungary.
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MeSH Terms
Cysteine / analysis,  metabolism*
Escherichia coli / genetics
Glutathione / analysis,  metabolism*
Nutritive Value
Plant Tubers / chemistry*
Plants, Genetically Modified / chemistry*
Serine O-Acetyltransferase / metabolism*
Solanum tuberosum / chemistry*,  genetics
Transformation, Genetic
Reg. No./Substance:
52-90-4/Cysteine; 70-18-8/Glutathione; EC O-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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