Document Detail

Improving nutritional quality of maize proteins by expressing sense and antisense zein genes.
MedLine Citation:
PMID:  15053536     Owner:  NLM     Status:  MEDLINE    
The predominant proteins in maize grain are a family of alcohol-soluble prolamin storage proteins called zeins. They account for >50% of total seed proteins but are deficient in several essential amino acids. As a result, the corn grain is considered to be nutritionally poor for monogastric animals with respect to key essential amino acids, most notably lysine, tryptophan, and methionine. Thus, corn mutants with reduced levels of zeins, such as opaque-2 (o2), have been demonstrated to possess grain with improved nutritional quality characteristics. The o2 mutant has a superior amino acid composition and has been used through conventional breeding to develop Quality Protein Maize (QPM) for human and animal consumption in developing countries. With the understanding of molecular genetics of zeins and progress in biotechnology, an alternative approach to zein reduction is explored here. Through the targeted reduction of the 19-kDa alpha-zeins, increased levels of lysine, tryptophan, and methionine have been engineered in grain of transgenic hybrids. Currently, the agronomic properties and nutritional values of these transgenic lines are being evaluated.
Shihshieh Huang; Whitney R Adams; Qing Zhou; Kathleen P Malloy; Dale A Voyles; Jan Anthony; Alan L Kriz; Michael H Luethy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  52     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-31     Completed Date:  2004-05-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1958-64     Citation Subset:  IM    
Mystic Research, Monsanto Company, 62 Maritime Drive, Mystic, Connecticut 06355, USA.
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MeSH Terms
DNA, Antisense / genetics*
Gene Expression*
Nutritive Value*
Plants, Genetically Modified / chemistry,  genetics*
Seeds / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Zea mays / chemistry,  genetics*
Zein / genetics*
Reg. No./Substance:
0/DNA, Antisense; 9010-66-6/Zein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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