Document Detail


Improving the long-term storage of a mammalian biosensor cell line via genetic engineering.
MedLine Citation:
PMID:  20178117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The unique properties of mammalian cells make them valuable for a variety of applications in medicine, industry, and diagnostics. However, the utility of such cells is restricted due to the difficulty in storing them non-frozen for an extended time and still maintaining their stability and responsiveness. In order to extend the active life span of a mammalian biosensor cell line at room and refrigerated temperatures, we have over expressed genes that are reported to provide protection from apoptosis, stress, or oxidation. We demonstrated that over expression of genes from the extremophile, Artemia franciscana, as well as GADD45beta, extends room-temperature storage of fully active cells 3.5-fold, while over production of several anti-apoptotic proteins extended 4 degrees C storage 2- to 3-fold. Methodologies like these that improve the stability of mammalian-cell-based technologies in the absence of freezers may enable widespread use of these tools in applications that have been considered impractical based solely on limited storage characteristics.
Authors:
Martha S Petrovick; Frances E Nargi; Theresa Towle; Kristine Hogan; Marie Bohane; Deborah J Wright; Thomas H MacRae; Malcolm Potts; Richard F Helm
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  106     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-03     Completed Date:  2010-07-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  474-81     Citation Subset:  IM    
Copyright Information:
2010 Wiley Periodicals, Inc.
Affiliation:
MIT Lincoln Laboratory, Lexington, Massachusetts 02420, USA. petrovick@ll.mit.edu <petrovick@ll.mit.edu>
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MeSH Terms
Descriptor/Qualifier:
Animals
Artemia / genetics
Biosensing Techniques*
Cell Line
Cell Survival
Gene Expression
Genetic Engineering*
Temperature
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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