| Improving lactate analysis with the YSI 2300 GL: hemolyzing blood samples makes results comparable with those for deproteinized whole blood. | |
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MedLine Citation:
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PMID: 1395001 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To obviate the well-documented problem of hematocrit dependency of the Yellow Springs Instruments (YSI) whole-blood lactate analyzer, we modified the dilution buffer by including a lysing reagent. This makes the results comparable with those of methods performed with deproteinized whole-blood samples. No centrifugation step is needed, thus preserving the convenience of the YSI instrument for stat and field use. The modification works equally well on plasma samples. Lactate concentrations measured in nonhemolyzed whole blood are not comparable with results for hemolyzed whole blood or protein-precipitated whole blood. We therefore recommend to all users of YSI equipment to lyse the erythrocytes before lactate determinations. |
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Authors:
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P Foxdal; Y Bergqvist; S Eckerbom; B Sandhagen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry Volume: 38 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1992 Oct |
Date Detail:
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Created Date: 1992-11-16 Completed Date: 1992-11-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2110-4 Citation Subset: IM |
Affiliation:
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Department of Clinical Physiology, University Hospital, Uppsala, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques*
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standards Blood Chemical Analysis / instrumentation*, standards Drug Stability Hemolysis* Humans Indicators and Reagents Lactates / blood* Lactic Acid Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Lactates; 50-21-5/Lactic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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