| Improving cell therapy--experiments using transplanted telomerase-immortalized cells in immunodeficient mice. | |
| | |
MedLine Citation:
|
PMID: 17123586 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cell therapy is the use of stem cells and other types of cells in various therapies for age-related diseases. Two issues that must be addressed before cell therapy could be used routinely in medicine are improved efficacy of the transplanted cells and demonstrated long-term safety. Desirable genetic modifications that could be made to cells to be used for cell therapy include immortalization with human telomerase reverse transcriptase (hTERT). We have used a model for cell therapy in which transplantation of adrenocortical cells restores glucocorticoid and mineralocorticoid hormone levels in adrenalectomized immunodeficient mice. In this model, clones of cells that had been immortalized with hTERT were shown to be able to replace the function of the animals' adrenal glands by forming vascularized tissue structures when cells were transplanted beneath the capsule of the kidney. hTERT-modified cells showed no tendency for neoplastic changes. Moreover, a series of experiments showed that hTERT does not cooperate with known oncoproteins in tumorigenesis either in adrenocortical cells or in human fibroblasts. Nevertheless, hTERT was required for tumorigenesis when cells were implanted subcutaneously rather than in the subrenal capsule space. Changes in gene expression make hTERT-modified cells more robust. Understanding these changes is important so as to be able to separately control immortalization and other desirable properties of cells that could be used in cell therapy. Alternatively, desirable properties of transplants might be provided by co-transplanted mesenchymal cells: mesenchymal cell-assisted cell therapy. For both hTERT modification and mesenchymal cell-assisted cell therapy, genomics approaches will be needed to define what genetic modifications are desirable and safe in cells used in cell therapy. |
| | |
Authors:
|
Qin Huang; Meizhen Chen; Sitai Liang; Victor Acha; Dan Liu; Furong Yuan; Christina L Hawks; Peter J Hornsby |
Related Documents
:
|
15126386 - Exo1 plays a role in generating type i and type ii survivors in budding yeast. 2919136 - Quantitative histology of cartilage cell columns in the human costochondral junction: f... 1372686 - Effects of mutagens on the clonal lifespan of paramecium tetraurelia. 6205166 - Antigenic structure of simian virus 40 large tumor antigen and association with cellula... 23505386 - Both the caspase csp-1 and a caspase-independent pathway promote programmed cell death ... 21456786 - A miniature cell for gas solubility measurements in oils and bitumen. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review Date: 2006-11-22 |
Journal Detail:
|
Title: Mechanisms of ageing and development Volume: 128 ISSN: 0047-6374 ISO Abbreviation: Mech. Ageing Dev. Publication Date: 2007 Jan |
Date Detail:
|
Created Date: 2007-01-15 Completed Date: 2007-04-20 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 0347227 Medline TA: Mech Ageing Dev Country: Ireland |
Other Details:
|
Languages: eng Pagination: 25-30 Citation Subset: IM |
Affiliation:
|
Department of Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78245, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Humans Immunologic Deficiency Syndromes / therapy* Mesenchymal Stem Cell Transplantation* Mesenchymal Stem Cells* / enzymology Mice Telomerase / genetics, physiology* |
| Grant Support | |
ID/Acronym/Agency:
|
AG12287/AG/NIA NIH HHS; AG20752/AG/NIA NIH HHS; P01 AG020752-020006/AG/NIA NIH HHS; P30 CA54174/CA/NCI NIH HHS; R37 AG012287-13/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
EC 2.7.7.49/Telomerase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Multivariant statistical analysis of PCDD/FS in sewage sludges from different areas of the Valencian...
Next Document: Chaperonomics, a new tool to study ageing and associated diseases.