| Improving Sperm Cryopreservation with Antifreeze Proteins: Effect on Gilthead Seabream (Sparus aurata) Plasma Membrane Lipids. | |
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MedLine Citation:
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PMID: 22088915 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Changes in the plasma membrane lipid composition have been related to a decrease in sperm quality during cryopreservation. Antifreeze proteins (AFPs) have been tested in different species because of their ability to depress the freezing point and their potential interaction with membranes, but controversial effects were reported. In the present study we analyzed separately the lipid composition of two sperm membrane domains, head (HM) and flagellar (FM) membrane, after cryopreservation with an extender containing 5% DMSO either alone or with AFPI or AFPIII (1 µg/ml). We used sperm from a teleost, Sparus aurata, because the lack of acrosome avoids changes of lipid profiles due to capacitation process or acrosomal losses during freezing/thawing. Comparing with the control (cryopreservation with 5% DMSO alone) the addition of AFPIII increased the velocity, linearity of movement and the percentage of viable cells. In addition, freezing with DMSO alone increased the phosphatidyl-serine content, as well as the saturated fatty acids and decreased the unsaturated ones (mainly PUFA) both in HM and FM. These changes in the lipid components were highly avoided with the addition of AFPIII. HM had a higher amount of saturated fatty acids than FM and was more affected by cryopreservation without AFPs. The percentage of viable cells was positively correlated with the amount of unsaturated fatty acids in the HM whereas the motility parameters were positively correlated with both FM and HM amount of unsaturated fatty acids. AFPs, especially AFPIII, seem to have interacted with unsaturated fatty acids stabilizing the plasma membrane organization during cryopreservation and contributing to improve sperm quality after thawing. |
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Authors:
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José Beirão; Loredana Zilli; Sebastiano Vilella; Elsa Cabrita; Roberta Schiavone; Maria Paz Herráez |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-16 |
Journal Detail:
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Title: Biology of reproduction Volume: - ISSN: 1529-7268 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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