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Improvements in ethanol tolerance of Kluyveromyces fragilis in jerusalem artichoke juice.
MedLine Citation:
PMID:  18584667     Owner:  NLM     Status:  In-Data-Review    
Alcoholic fermentation of Jerusalem artichoke juice, a natural complex medium, allowed the production of 13% (v/v) ethanol utilizing an inulin-fermenting strain of Kluyveromyces fragilis, strongly sensitive to ethanol. However, the fermentation of a simple medium with a similar concentration of fermentable sugars (235 g/L) as saccharose stopped prematurely when only 7% (v/v) ethanol had been produced. Differences in the two fermentation profiles were attributed to the significantly lower ethanol tolerance of K. fragilis IGC 2671 in the simple medium with 2% saccharose as compared with diluted J.a. juice with a similar sugar concentration, in fact, (1) in diluted J. a. juice, growth was possible up to 8% (v/v) added ethanol compared with 6% (v/v) in simple medium and (2) ethanol-induced inhibition of the specific growth and fermentation rate as well as ethanol-induced stimulation of the specific death rate were much more drastic in simple medium. Present results show that (1) the complex composition of the medium used for alcoholic fermentation plays a marked role in the ability of the yeast to tolerate and produce ethanol; (2) J. a. juice proved a very appropriate medium for a productive alcoholic fermentation, namely, in processes based on strains with a low ethanol resistance; and (3) to characterize and compare the ethanol tolerance of fermenting yeasts, the standardization of the medium composition must be taken in consideration.
M F Rosa; I S Correia; J M Novais
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  31     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  1988 May 
Date Detail:
Created Date:  2008-06-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  705-10     Citation Subset:  -    
Departamento de Energias Renováveis, Laboratório Nacional de Engenharia e Tecnologia Industrial, 1699 Lisboa, Codex, Portugal.
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