Document Detail

Improvement of steroid biotransformation with hydroxypropyl-beta-cyclodextrin induced complexation.
MedLine Citation:
PMID:  19189059     Owner:  NLM     Status:  MEDLINE    
The inclusion complexes induced by cyclodextrins and its derivates have been shown previously to enhance the biotransformation of hydrophobic compounds. Using hydroxypropyl-beta-cyclodextrin (HP-beta-CD; 20% w/v), the water solubility of cortisone acetate increased from 0.039 to 7.382 g L(-1) at 32 degrees C. The solubilization effect of HP-beta-CD was far superior to dimethylformamide (DMF) and ethanol. The dissolution rate also significantly increased in the presence of HP-beta-CD. The enzymatic stability of Delta(1)-dehydrogenase from Arthrobacter simplex TCCC 11037 was not influenced by the increasing concentrations of HP-beta-CD contrary to the organic cosolvents which negatively influenced in the order DMF > ethanol. The activity inhibition effect caused by HP-beta-CD was not so conspicuous as ethanol and DMF. Inactivation constants of ethanol, DMF, and HP-beta-CD were 5.832, 4.541, and 1.216, respectively. The inactivation energy (E (a)) was in the order of HP-beta-CD (55.1 kJ mol(-1)) > ethanol (39.9 kJ mol(-1)) > DMF (37.1 kJ mol(-1)).
Liting Zhang; Min Wang; Yanbing Shen; Yinhu Ma; Jianmei Luo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-03
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  159     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-10-23     Completed Date:  2010-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  642-54     Citation Subset:  IM    
Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
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MeSH Terms
Arthrobacter / enzymology,  metabolism*
Dimethylformamide / pharmacology
Enzyme Stability / drug effects
Ethanol / pharmacology
Oxidoreductases / metabolism
Steroids / chemistry*,  metabolism*
beta-Cyclodextrins / pharmacology*
Reg. No./Substance:
0/Steroids; 0/beta-Cyclodextrins; 64-17-5/Ethanol; 68-12-2/Dimethylformamide; 94035-02-6/2-hydroxypropyl-beta-cyclodextrin; EC 1.-/Oxidoreductases; EC delta(1) dehydrogenase

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