| Improvement of steroid biotransformation with hydroxypropyl-beta-cyclodextrin induced complexation. | |
MedLine Citation:
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PMID: 19189059 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The inclusion complexes induced by cyclodextrins and its derivates have been shown previously to enhance the biotransformation of hydrophobic compounds. Using hydroxypropyl-beta-cyclodextrin (HP-beta-CD; 20% w/v), the water solubility of cortisone acetate increased from 0.039 to 7.382 g L(-1) at 32 degrees C. The solubilization effect of HP-beta-CD was far superior to dimethylformamide (DMF) and ethanol. The dissolution rate also significantly increased in the presence of HP-beta-CD. The enzymatic stability of Delta(1)-dehydrogenase from Arthrobacter simplex TCCC 11037 was not influenced by the increasing concentrations of HP-beta-CD contrary to the organic cosolvents which negatively influenced in the order DMF > ethanol. The activity inhibition effect caused by HP-beta-CD was not so conspicuous as ethanol and DMF. Inactivation constants of ethanol, DMF, and HP-beta-CD were 5.832, 4.541, and 1.216, respectively. The inactivation energy (E (a)) was in the order of HP-beta-CD (55.1 kJ mol(-1)) > ethanol (39.9 kJ mol(-1)) > DMF (37.1 kJ mol(-1)). |
Authors:
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Liting Zhang; Min Wang; Yanbing Shen; Yinhu Ma; Jianmei Luo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-03 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 159 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-10-23 Completed Date: 2010-01-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 642-54 Citation Subset: IM |
Affiliation:
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Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arthrobacter
/
enzymology,
metabolism* Dimethylformamide / pharmacology Enzyme Stability / drug effects Ethanol / pharmacology Oxidoreductases / metabolism Steroids / chemistry*, metabolism* beta-Cyclodextrins / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Steroids; 0/beta-Cyclodextrins; 64-17-5/Ethanol; 68-12-2/Dimethylformamide; 94035-02-6/2-hydroxypropyl-beta-cyclodextrin; EC 1.-/Oxidoreductases; EC 1.3.99.4/3-oxosteroid delta(1) dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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