| Improvement of polyunsaturated fatty acids synthesis by the coexpression of CYB5 with desaturase genes in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 20512324 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Since Saccharomyces cerevisiae contains Delta9 fatty acid desaturase (OLE1) as a sole fatty acid desaturase, it produces saturated and monounsaturated fatty acids of 16- and 18-carbon compounds. We showed earlier that Kluyveromyces lactis Delta12 (KlFAD2) and omega3 (KlFAD3) fatty acid desaturase genes enabled S. cerevisiae to make also polyunsaturated fatty acids (PUFAs), linoleic (18:2n-6), and alpha-linolenic (18:3n-3) acids. Unlike Delta9 fatty acid desaturase Ole1p, the two added fatty acid desaturases (KlFAD2and KlFAD3) do not contain a cytochrome b5 domain, and we now report on effects of the overexpression of K. lactis and S. cerevisiae cytochrome b5 (CYB5) genes as well as temperature effects on PUFA synthesis. Without extra cytochrome b5, while PUFA synthesis is significant at low temperature (20 degrees C), it was marginal at 30 degrees C. Overexpression of cytochrome b5 at 20 degrees C did not affect the fatty acid synthesis so much, but it significantly enhanced the synthesis of PUFA at 30 degrees C. |
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Authors:
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Hisashi Yazawa; Hitoshi Iwahashi; Yasushi Kamisaka; Kazuyoshi Kimura; Hiroshi Uemura |
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Publication Detail:
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Type: Journal Article Date: 2010-05-29 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 87 ISSN: 1432-0614 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 2185-93 Citation Subset: IM |
Affiliation:
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Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central, Ibaraki, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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