| Improvement of the performance of H2O2 oxidation at low working potential by incorporating TTF-TCNQ into a platinum wire electrode for glucose determination. | |
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MedLine Citation:
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PMID: 10230033 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A micro-biosensor was constructed by incorporating the organic conducting salt tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ) into a platinized platinum (Pt) wire and further covering with the electrochemical polymerical heteropolypyrrole film, in which glucose oxidase (GOx) was entrapped. The enzyme electrode can sensitively determine glucose at a low working potential, mainly based on the oxidation of H2O2. The incorporated TTF-TCNQ can significantly improve the oxidation of H2O2 on the electrode, although a part of the TTF-TCNQ functions as a mediator. Compared with the same electrode prepared without TTF-TCNQ incorporated, the TTF-TCNQ modified electrode had better performance characteristics at a working potential of 200 mV (versus SCE). The response time to 90% of the steady value was shortened from about 40 s to less than 10 s, the lower limit of the linear response was greatly extended from about 1.6 mM to 10 microM, the linear range was shifted from 1.6-10.0 to 0.01-5 mM and the sensitivity was increased from about 1 to 1.5 microA/mM. The electrode was quite stable. For continuous operation, the electrode could work for about 5 weeks and only lost 60% of its original sensitivity. Stored at 4 degrees C for intermittent determinations, the electrode kept 80% sensitivity for over 6 months. Due to covering the electrode with a non-conductive heteropolypyrrole film, ascorbate, urate and 4-acetamidophenol caused only negligible current response at an applied potential of 200 mV. |
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Authors:
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Q S Li; B C Ye; B X Liu; J J Zhong |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 14 ISSN: 0956-5663 ISO Abbreviation: Biosens Bioelectron Publication Date: 1999 Mar |
Date Detail:
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Created Date: 1999-06-24 Completed Date: 1999-06-24 Revised Date: 2009-07-14 |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 327-34 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques* Electrochemistry Electrodes Electron Transport Enzyme Stability Glucose / analysis* Glucose Oxidase Heterocyclic Compounds* Hydrogen Peroxide / chemistry* Indicators and Reagents Nitriles* Oxidation-Reduction Oxygen / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Heterocyclic Compounds; 0/Indicators and Reagents; 0/Nitriles; 1518-16-7/tetracyanoquinodimethane; 31366-25-3/tetrathiafulvalene; 50-99-7/Glucose; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen; EC 1.1.3.4/Glucose Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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