Document Detail

Improvement of the performance of H2O2 oxidation at low working potential by incorporating TTF-TCNQ into a platinum wire electrode for glucose determination.
MedLine Citation:
PMID:  10230033     Owner:  NLM     Status:  MEDLINE    
A micro-biosensor was constructed by incorporating the organic conducting salt tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ) into a platinized platinum (Pt) wire and further covering with the electrochemical polymerical heteropolypyrrole film, in which glucose oxidase (GOx) was entrapped. The enzyme electrode can sensitively determine glucose at a low working potential, mainly based on the oxidation of H2O2. The incorporated TTF-TCNQ can significantly improve the oxidation of H2O2 on the electrode, although a part of the TTF-TCNQ functions as a mediator. Compared with the same electrode prepared without TTF-TCNQ incorporated, the TTF-TCNQ modified electrode had better performance characteristics at a working potential of 200 mV (versus SCE). The response time to 90% of the steady value was shortened from about 40 s to less than 10 s, the lower limit of the linear response was greatly extended from about 1.6 mM to 10 microM, the linear range was shifted from 1.6-10.0 to 0.01-5 mM and the sensitivity was increased from about 1 to 1.5 microA/mM. The electrode was quite stable. For continuous operation, the electrode could work for about 5 weeks and only lost 60% of its original sensitivity. Stored at 4 degrees C for intermittent determinations, the electrode kept 80% sensitivity for over 6 months. Due to covering the electrode with a non-conductive heteropolypyrrole film, ascorbate, urate and 4-acetamidophenol caused only negligible current response at an applied potential of 200 mV.
Q S Li; B C Ye; B X Liu; J J Zhong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  14     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-06-24     Completed Date:  1999-06-24     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  327-34     Citation Subset:  IM    
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People's Republic of China.
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MeSH Terms
Biosensing Techniques*
Electron Transport
Enzyme Stability
Glucose / analysis*
Glucose Oxidase
Heterocyclic Compounds*
Hydrogen Peroxide / chemistry*
Indicators and Reagents
Oxygen / pharmacology
Reg. No./Substance:
0/Heterocyclic Compounds; 0/Indicators and Reagents; 0/Nitriles; 1518-16-7/tetracyanoquinodimethane; 31366-25-3/tetrathiafulvalene; 50-99-7/Glucose; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen; EC Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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