Document Detail


Improvement in the in vitro maturation rate of porcine oocytes vitrified at the germinal vesicle stage by treatment with a mitochondrial permeability transition inhibitor.
MedLine Citation:
PMID:  18838069     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we examined the effects of inhibitors of mitochondrial permeability transition (MPT), caspase activity, intracellular Ca(2+) chelator and mitochondrial Ca(2+) uniporter on survival assessed by morphological observation and in vitro maturation (IVM) of porcine vitrified germinal vesicle (GV) oocytes. When vitrified GV oocytes were matured only present in the IVM medium with an MPT inhibitor, cyclosporin A (CsA), the survival and IVM rates (36.1% and 26.8%, respectively) were significantly higher (P<0.05) than those in the other vitrified groups (10.3-12.3% and 6.2-10.3%, respectively). However, Z-VAD-fmk (Z-VAD), a caspase inhibitor, did not improve the survival and IVM rates (11.7-21.6% and 8.5-155%, respectively). When BAPTA-AM, an intracellular Ca(2+) chelator, was present in the IVM medium, the survival and IVM rates of vitrified GV oocytes (34.5-36.2% and 25.0-26.9%, respectively) were significantly higher (P<0.05) than those in the absent vitrified groups (17.2-24.2% and 12.9-19.3%, respectively). When ruthenium red (RR), an inhibitor of mitochondrial Ca(2+) uniporter, was present only in the IVM medium, the survival and IVM rates (54.5% and 39.4%, respectively) were significantly higher than those in the other vitrified groups (25.8-38.4% and 14.4-24.2%, respectively). Furthermore, blastocysts were successfully produced using porcine vitrified GV oocytes matured in the IVM medium with RR after in vitro fertilization. These results suggested that CsA, BAPTA-AM and RR but not Z-VAD have improved the survival and IVM rates of porcine vitrified GV oocytes.
Authors:
Shoma Nakagawa; Akihiro Yoneda; Koji Hayakawa; Tomomasa Watanabe
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Publication Detail:
Type:  Journal Article     Date:  2008-09-22
Journal Detail:
Title:  Cryobiology     Volume:  57     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-01     Completed Date:  2009-01-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  269-75     Citation Subset:  IM    
Affiliation:
Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Chloromethyl Ketones / pharmacology
Animals
Cell Survival
Cells, Cultured
Chelating Agents / pharmacology
Cryopreservation / methods*
Cryoprotective Agents / pharmacology*
Cyclosporine / pharmacology
Cysteine Proteinase Inhibitors / pharmacology
Egtazic Acid / analogs & derivatives,  pharmacology
Enzyme Inhibitors / pharmacology
Female
Metaphase
Mitochondrial Membrane Transport Proteins / antagonists & inhibitors
Oocytes / cytology*,  drug effects*
Ruthenium Red / pharmacology
Swine
Chemical
Reg. No./Substance:
0/Amino Acid Chloromethyl Ketones; 0/Chelating Agents; 0/Cryoprotective Agents; 0/Cysteine Proteinase Inhibitors; 0/Enzyme Inhibitors; 0/Mitochondrial Membrane Transport Proteins; 0/benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; 0/mitochondrial permeability transition pore; 11103-72-3/Ruthenium Red; 139890-68-9/1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; 59865-13-3/Cyclosporine; 67-42-5/Egtazic Acid

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