| Improvement of cell viability and hirudin production by ascorbic acid in Pichia pastoris fermentation. | |
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MedLine Citation:
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PMID: 16525778 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In recombinant Pichia pastoris fermentation for hirudin production, copious cells were not viable and most of the secreted hirudin molecules were C-terminally truncated at the end of fermentation. In this work, the influences of reactive oxygen species (ROS) on cell viability and hirudin production were subsequently studied. In contrast to the untreated control condition, the addition of ascorbic acid at the methanol fed-batch phase could obviously relieve the damage of intracellular ROS to cell membranes. As a result, the cell viability could be increased to 91% from 74% in control at the end of fermentation and the extracellular proteolysis of hirudin reduced. Intact and total hirudin production, by supplying ascorbic acid, could reach 2.90 and 5.03 g/l, respectively, in contrast to 1.75 and 4.70 g/l at the control condition. Ascorbic acid, 4 mmol/l or more, in the fermentation broth increased markedly the production of the intact hirudin, despite a little effect on total hirudin production. |
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Authors:
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Anfeng Xiao; Xiangshan Zhou; Li Zhou; Yuanxing Zhang |
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Publication Detail:
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Type: Journal Article Date: 2006-03-09 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 72 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-09-26 Completed Date: 2007-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 837-44 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Ascorbic Acid
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metabolism* Cell Survival Fermentation Hirudins / biosynthesis* Pichia / classification, metabolism*, physiology |
| Chemical | |
Reg. No./Substance:
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0/Hirudins; 50-81-7/Ascorbic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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